| Objectives: By setting up intestine ischemia-reperfusion model rats, to investigate the effects and the mechanism of Taohechengqi decoction (THCQ) pretreating rats with intestine ischemia-reperfusion. Especially on intestinal mucosa cell apoptosis and its relevent genes expression. Supply experiment and theory basis for TCM treating intestine ischemia-reperfusion, which reveal the basic rule of TCM on treating the disease.Methods: In experiment, 56 wistar rats were randomly divided into 7 groups. Every group had 8 rats (male and female by half). The first group is normal group, the second is sham group, the third is intestine ischemia-reperfusion group, the forth is large dose THCQ decoction pretreatment group, the fifth is middle dose THCQ decoction pretreatment group, the sixth is small dose THCQ decoction pretreatment group, the last is lactulase control group. Except normal group and sham group, the other 5 groups were clapped with clippers in superior mesenteric artery to set up intestine I/R model. To explore the effect of THCQ decoction on rats' function of intestine, liver, kidney with intestine ischemia-reperfusion damage, we use biochemical ways to test plasma endotoxin, serum DAO, ALT,BUN changes. To explore the effect of THCQ decoction on rats' intestinal membrane barrier with intestine ischemia-reperfusion damage, we observe intestine tissue pathologic section, intestinal mucous damage score and intestinal mucosa cell ultramicrostructure. To explore the mechanism of THCQ decoction on rats' with intestine ischemia-reperfusion damage, we observe the intestinal mucosa cell apoptosis and its relevent genes JNK,Bcl-2, Caspase-3,NF-kB Protein expression.Results:(1)After intestine ischemia-reperfusion, the amount of plasma endotoxin and serum DAO, ALT,BUN had increased. Compwered with normal group and sham group, there were significantly difference than other five groups (P＜0.01). It shows the model is successful. The amount of plasma endotoxin and serum DAO, ALT,BUN had lowered in three THCQ decoction groups, lactulase control group than model group. There are significantly difference between them (P＜0.05). It shows that three dosages of THCQ decoction and lactulose can cure intestine ischemia-reperfusion of rats. Compared with each two groups, large dose THCQ decoction pretreatment group was superior to lactulase control group (P＜0.01 or P＜0.05).(2) After intestine ischemia-reperfusion, The damage of intestinal mucous was serious through observing intestinal histopathology and ultramicrostructure, and the damage of intestinal mucous score had obviously increased (P＜0.01). THCQ decoction and lactulose had obviously lowered the damage of intestinal mucous score (P＜0.01), and chang the abnomal intestinal histopathology and ultramicrostructure. Compared with each two groups, large dose THCQ decoction pretreatment group was equal to lactulase control group in treatment (P＞0.05). Large dose THCQ decoction pretreatment group was obviously superior to small and middle dose THCQ decoction pretreatment group (P＜0.01).(3) After intestine ischemia-reperfusion, the intestinal mucosa cell apoptosis index and the amount of JNK protein had obviously risen up (P＜0.05). After adding medicine, the intestinal mucosa cell apoptosis index and the amount of JNK protein had obviously lowered (P＜0.05) It shows that THCQ decoction can restrict JNK acting and reduce the intestinal mucosa cell apoptosis. Compared with each two groups, Large dose THCQ decoction pretreatment group was the best (P＜0.05).(4) After intestine ischemia-reperfusion, the intestinal mucosa cell Caspase-3,NF-kB genes Protein expression had obviously risen up (P＜0.05), the amount of Bcl-2 protein had obviously lowered (P＜0.05). After adding medicine, the intestinal mucosa cell Caspase-3,NF-kB genes Protein expression had obviously lowered (P＜0.05), the amount of Bcl-2 protein had obviously risen up (P＜0.05). It shows that THCQ decoction can restrict Caspase-3,NF-kB acting and enhance Bcl-2 gene to reduce the intestinal mucosa cell apoptosis. Compared with each two groups, Large dose THCQ decoction pretreatment group was the best (P＜O.05).Conclusions:(1)The model is successful. (2)Each dose of THCQ decoction pretreatment group can pretreat the intestine ischemia-reperfusion of rats, which large dose THCQ decoction pretreatment group is the best. It shows that the effect is related to the dosage. (3)THCQ decoction can lower the level of the amount of plasma endotoxin and serum DAO, ALT, BUN. (4)THCQ decoction can lower the intestine tissue pathological and intestinal mucosa cell ultramicrostructure changes, lower the damage of intestinal mucous score, keep the intestinal mucous integrity and protect the intestinal barrier. (5)THCQ decoction can reduce the intestinal mucosa cell apoptosis after intestine ischemia-reperfusion. (6)THCQ decoction can restrict JNK/MAPK acting. (7)THCQ decoction can restrict Caspase-3, NF-kB acting and enhance Bcl-2 gene. |
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