Coptis Ginseng Drugs In Type 2 Diabetes Insulin Resistance Mechanism

On basis of clinical treatment of insulin resistance (IR) in type 2 Diabetes with Tradition Chinese Medicine(TCM)and Material medica, this study applies Neijing theory"Excessive fire consuming qi"into the clinical and laboratory researches and points out that there is fire consuming qi in the pathogenesis of IR of type 2 Diabetes , therefore purging fire together with invigorating qi can be used to treat it, according to"treating heat with cold". In this study, we observed the effect of huanglian (Rhizoma Coptidis)-renshen (Radix Ginseng)-pair on IR and secondaryβcell function damage in mice with laboratory type 2 diabetes to explore its pathogenesis and treatment and thus provide foundation for innovation in TCM theory on treating IR with Traditional Chinese Medicine and material medica.1. Literature studies: All recent relevant literature concerning Type 2 diabetes, IR, PPARγand animal experiments are documented. So this study has taken a full account of the mechanism, experimental and clinical studies of IR in type 2 diabetes into consideration both in TCM and allopathic medicine and made a systematic review of PPARγadvancements and animal experiments.2. Theoretical Studies: Under the guidance of TCM theory, we maintain that the treatment of IR is decisive in TCM treatment of type 2 diabetes. After reviewing the status quo of the clinical application of purging fire together with invigorating qi in treating IR, we elucidate the TCM pathogenesis of IR in type 2 diabetes and put forward the hypothesis that there is excessive fire consuming qi in the pathogenesis of IR, and that we should combine purging fire with invigorating qi in IR treatment. We, however, consider it more significant to explore effective clinical treatment of IR rather than theoretical studies to enrich TCM theory of IR in type 2 diabetes.3. Laboratory studiesObjectives: To observe the effects of huanglian-renshen-pair on laboratory IR mice with type 2 diabetes and their mechanisms.Methods: We rendered mice insulin resistant and diabetic by feeding high glucose and fat diet together with peritoneal injection of STZ and then treated it with huanglian-renshen-pair, comparing to Pioglitazone. Then we observed the effects of the drugs on glycometabolism, lipid metabolism, insulin sensitivity and pancreas pathomorpholgoy, serum TNFα, FFA and genetic expression of PPARγin IR mice.Results:①Evaluation of animal model: We succeeded in rendering laboratory IR of type 2 diabetes, manifested as hyperglycemia, hyperinsulinemia,...