| Vascular dementia(VD) has become a fredquently encountered disease and one of the main styles of the aged dementias because of the high incidence of cerebrovascular disease.The modern medicine has maken great development in the pathology and pathology-biology research on VD,many researchs in different angles and different levels have been made on VD.Recently, as more and more basic and clinic researchs on Traditional Chinese Medicine(TCM) treating VD have been made,more and more privileges have appeared. In summarise of this thesis I discussed the pathogeny,types and the treatments of VD in TCM's angle of view.Combining the clinical experience of my tutor ,I put forward that " positive Qi become weak and toxic Qi invade "is the pathogeny of VD,"increase the positive Qi and decrease the toxic Qi"is the rule and method of the treatment to VD,"ShenZhiJianNaoFang"is the appearance of the rule and method of "increase the positive Qi and decrease the toxic Qi",suit to the modern pharmacology research on VD.From the angle of modern medicine ,I researched the dangerous acts , pathology changes , diagnose and treatment of VD and the acethylcholine systeme's control action ,make the conclusion that acethylcholine systeme make a great role to the happenness and development of VD,we can reach the aim of treat and prevent VD by decrease the dangerous acts,control and improve the vascular occlusion etc. The experiment part is departed into four parts: Part one :Behavior part.â‘ Make the vascular dementia model by repeated close and open the rat's common carotid arterys in both sides and intraperitoneal inject sodium nitroprusside(SNP), using Morris water-mazed palace to test the rat's ability of study and memory and prove the succession of making the model.â‘¡Treat the model rats with large,medium,small dose of ShenZhiJianNaoFang and use Morris water-mazed palace to test the effection of ShenZhiJianNaoFang and make sure of the best dose. Part two:Pathology part. â‘ Divid the rats into controlled group,model group,positive drug group,ShenZhiJianNaoFang large,medium,small dose group. Make all the rats'pathology slices and dye in HE dying methods.Observe the model's change in CA1 district in hippocampus and the treatment's effection of the medicines.â‘¡Take count of the cells in CA1 district in hippocampus of all the HE dyed slices and compare it.â‘¢Make all the rats'pathology slices and dye in Nissle's dying methods.Observe the model's change in CA1 district in hippocampus and the treatment's effection of the medicines.â‘£Take count of the cells in CA1 district in hippocampus of all the Nissle's dyed slices and compare Part three:Part of acethylcholine (ACh)system.â‘ Make pathology slices of the rats in the controlled group,model group and ShenZhiJianNaoFang medium dose group.Observe the appearance of cholinacetyltranslase(ChAT) in CA1 district in hippocampus in immuo-histochemical method.â‘¡Make pathology slices of the rats in the controlled group , model group and ShenZhiJianNaoFang medium dose group.Observe the appearance of acethylcholine esterase(AChE) in CA1 district in hippocampus in immuo-histochemical method. â‘¢ Observe the appearance of acethylcholine (ACh) in hippocampus of controlled group , model group ,ShenZhiJianNaoFang medium dose group's rats by high performance liquid chromatograph-electrochemical detector.â‘£Detect the pathology changes of VD and ShenZhiJianNaoFang's treating effection through acethylcholine system'change. Discuss the principle of"positive Qi become weak"in VD's occur and the principle of "increase the positive Qi"in VD's treatment. Part four:Part of Bax, the protein associated with apoptosis. Make pathology slices of the rats in the controlled group,model group and ShenZhiJianNaoFang medium dose group.Observe the appearance of Bcl-2 associated X protein(Bax) in CA1 district in hippocampus in immuo-histochemical method.Discuss the principle of"toxic Qi invade"in VD's occur and the principle of "decrease the toxic Qi"in VD's treatment. Result:C...
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