Toxic-pathologic Trial Study On Cerebral Regions Related To Morphine Dependent Rat

Objective:To establish models of morphine dependent rats, observe histopathologic and ultrastructural pathologic changs in cerebral regions related to morphine dependent rats, then investigate relationship between toxic- pathologic changs of cerebral regions related to dependence and function metabolism. Methods: Models of morphine dependent rats were established by back hypodermic injection. Nerve cells and fibers, axons,dendrites and synapses of seven cerebral regions related to dependence Which were locus coeruleus, periaquedutal gray, substanti anigra, lenticula, corpus amygdaloideun, hippocampus and frontalis cortex of morphine dependent rats were observed by optical microscope and transmission electron microcope(TEM), and the results were compared with those of control group. The number of synapses were also compared one another by statistical method. Results:Rats of morphine dependent group appeared conspicuous withdrawal symptoms. The major histopathologic changs of seven dependent related regions in dependent groups were as follow:nerve cells pyknosis or swelling, nervous fibers swelling, gliocytes and sub-mater lymphocytes and monocytes hyperplasia which became more apparent as dependent time prolonged, and gliocyte tubers forming. Under TEM, mitochondria were swelled and deformed, endoplasmic reticulums were dilated, polyribosomes were disaggregated, the number of axons and dentrites as well as synapses were increased, moreover increasing synapses got more obvious as dependent time prolonged. As to synapses increasing, there was significantly difference between dependent group of 8 or 4 weeks and control group, and it was significantly different between dependent group of 8 weeks and dependent group of lor 2 week. Cone I usion:The models of morphine dependent rats are established by back hypodermic injection morphine. Morphine may result in nerve cells ischemic-anoxic and degradation changes, gliocytes and sub-mater lymphocytes as well as monocytes hyperplasia in different degree, synaptic vesicles increasing and accumulation in synaptic junctions, synapsesincreasing, and those changes are correlated to dependent time. The toxic-pathologic changes of cerebral regions related to dependence of morphine dependent rats are correlated to function metabolism.