| Objectives:To establish the physical-sign-collecting form and its collection methods; To explore the method of estimating the syndrome of animal models combining disease with syndrome of Type 2 DM, which are duplicated with high-fat diet and Streptozotocin (STZ); To evaluate the curative effect of formulas on animal models combining disease with syndrome of Type 2 DM; To explore the essence of syndrome of animal models and the mechanism of curative effect of formulas by means of urine metabonomics.Methods:The research on physical signs and syndrome of diabetic rat models built up with STZ was carried out, the physical-sign-collecting form and its collection method were established. Blood glucose, blood lipid, hormone, body temperature, body weight and so on were measured. The diagnosis of syndrome of rat models was made in the basis of combination of diagnostic standard of clinical syndrome and viewpoints of specialists; The research on physical signs and syndrome of diabetic rat models built up with high-fat diet and STZ was carried out. The physical signs were collected according to the former physical-sign-collecting form. Blood glucose, blood lipid, hormone and so on were measured. The diagnosis of syndrome of rat models was made in the same method; The research on evaluating the curative effect of formulas applied on rat models built up with high-fat diet and STZ was carried out. The estimation of curative effect of formulas was made by means of physical-sign-collection and measurement of blood glucose, blood lipid and pathological changes; The research on applying GC-MS to analyzing the urine of formula-treating rat models built up with high-fat diet and STZ was carried out. The differences of metabolites in different groups and time were analyzed and explained the biological basis of syndrome and mechanism of curative effect. Results:The physical-sign-collecting form and its collection method were established through the research on physical signs and syndrome of diabetic rat models built up with STZ; On the basis of collecting physical signs and measuring blood, the method of diagnosing syndrome of rat models built up with high-fat diet and STZ had been established according to the combination of diagnostic standard of clinical syndrome and viewpoints of specialists. In the former 3 weeks, compared with control group, the physical signs such as slim shape, dull action, yellow fur, white ears, nose, slip and tongue had statistical significance; Blood glucose and hemorheology increased significantly; the body weight and strength of grabbing decreased significantly, the intake of water and food, the volume of urine and body temperature did not have statistical significance. In the latter 3 weeks, sticky and loose stool and heavy-red-color tongue increased significantly; After 6 weeks of oral intake of formulas, compared with model group, the physical signs had statistical significance, such as slim shape, dull action, white ears, nose, slip and tongue, especially in the latter 3 weeks. The blood glucose, blood lipid, liver function and renal function also had statistical significance; Compared with the same group in the former 3 weeks, the metabolites in urine of model group had statistical significance, including dihydroxybutanoic acid, L-ascorbic acid, D-gluconic acid, octadecanoic acid, pentanedioic acid. Compared with the same group in the former 3 weeks, L-ascorbic acid had decreased significantly in the latter 3 weeks in the formulas group.Conclusion:The physical-sign-collecting form could be a suitable instrument for collecting physical signs totally. The physical signs of diabetic rat models built up with STZ are stable through the 3rd to 7th weeks. The syndrome can be diagnosed as the syndrome of stomach heat at first and spleen deficiency at latter, but the syndrome is not found commonly in clinics; the physical signs of diabetic rat models induced by high fat diet and STZ are stable through the 1st to 6th weeks. The syndrome of rat models may be diagnosed as the syndrome of spleen deficiency and intermingled phlegm and blood stasis, which is often found in clinics. The kind of rat models may be qualified for research of verifying syndrome by corresponding formulas; the formulas showed its effects on the physical signs and laboratory items. The evaluation of curative effects could be carried out by estimating physical signs and changes of laboratory items; the changes of metabolites in urine may explain the mechanism of syndrome and curative effects of formulas. |
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