Somatostatin (SS) Involved In The Inflammatory Pain And Electro-Acupuncture Analgesia And Immunoregulation In AA Rats

Objective: Studying the influence of somatostatin ( SS ) on FAC-induced hyperalgesia, acupuncture analgesia and immunity, to explore the influence of SS on the effect of acupuncture analgesia and acupuncture regulation of immunity and their possible conjunct mechanism.Methods: The study was designed to observe SS and influence of serum CD₄⁺,CD₈⁺,IL-2,IL-6,TNF-α, splenic lymphocyte transformation rate, the expression of SS,SSTR-2 in spinal cord,PAG,NRM and hypothalamus the coexistence of SS and enkephalinergic neuron in back corner of spinal cord, the coexistence of SS and 5-HT neuron in NRM by using methods of behavioral nociceptive threshold test, measure of foot's cubage, flowcytometry, MTT, radioimmunity, HPLC-ECD, immunohistochemistry, laser confocal microscope on FAC-induced hyperalgesia.Results:Using intrathecal injection and electro-acupuncture, rats'pain threshold can be enhanced, secrum CD₄⁺,CD₈⁺,IL-2,LTT be improved, IL-6,TNF-αbe decreased, the expression of SS,SSTR-2 in spinal cord,PAG,NRM and hypothalamus be increased, SS and enkephalin or 5-HT in spinal cord or NRM be coexisted.Conclusion: SS may be an important regulative point on acupuncture analgesia and regulation of immunity.