An Experimental Study On The Construction Of PIRES-CD,pIRES-TK And The Double Suicide Gene Therapy Of Adenoid Cystic Carcinoma

Objective Adenoid cystic carcinoma, which is also called cylindroma,was first reported by Billroth. Most scholar considered that it comes from salivary ducts,or probably the oral squamous basal cells. It characterizes by encroaching the nerve and far matastasis. The treatment of adenoid cystic carcinoma gives priority to operation combined with post operation irradiation and chemistry therapy,but the curative effect is still dissatisfactory. Therefore,it makes sense to explore new therapy.Recently, the gene therapy of malignant tumor is developed rapidly and there are many methods about the gene therapy that include: suicide gene therapy, immunologic gene therapy, drug resistance gene therapy, angiostatin gene therapy and so on. The sucide gene therapy is one of the most potential approach of antitumor. Suicide gene therapy which is also named enzyme-prodrug therapy, encode enzyme that convert nontoxic prodrugs into highly toxic metablites .Cells transfected with suicide genes are targeted for specific negative selection,witch can be induced by administrtion of the corresponding produg. Among the enzyme/produg combinations,two of the best characterized system are herpes simplex virus thymidine kinase(TK)/ganciclovir(GCV) and Escherichia Foli cytosine deaminase (CD) /5-flourocytosine(5-FC).The formor can convert the antiviral nucleoside analogs acyclovir(ACV),gancielovir(GCV) to their nucleoside monophosphate derivatives,the monophosphate forms are subsequently phosphorylated by endogenus cellular kinases to triphosphates, these molecules are potent inhibitors of DNA synthesis ; The later...