Adenoid Cystic Carcinoma Of High And Low Metastatic Cell Line Gene Expression Differences And B7-1 Of Xage-1b Transfer Correlation Study

The molecular genesis of a tumor is a multi-step and complex process in which many factors are involved such as oncogenes , tumor suppressor genes and MHC-â…  etc. Metastasis is the essential character causing mortality f rom such tumors and little is currently known about mechanisms underlying cancer metastasis. As a step toward understanding the complex differences between high metastatic and low metastatic cells, metastasis2associated gene expression patterns and proteomes were separated and identified by suppression subtraction hybridization and comparative proteomics analysis between high metastatic cell ACCM and low metastatic cell ACC2. Although extensive similarity was noted between the expression profiles of the two cell lines, twenty-eight genes highly expressed were obtained in tester, low metastatic cells , compared with their low expression in driver , high metastatic cells while six genes in reverse hybridization. These function of genes are related to cell proliferation, differentiation or cell metabolism while several novel EST sequences which function are unknown. Two novel genes were ACC metastasis-associated RNH ACC metastasis associated suspected protein. Meanwhile, ten proteins were randomly selected which are expressed different qualitatively or quantitatively and identified by mass fingerprinting ( MALDI-TOF-MS) . These proteins are st ratifin , symplekin , B7-1 , TPMsk1 t ropomyosin isoforms , serologically defined breast cancer antigen N Y2BR220 etc. Intriguingly , most identified candidate proteins have been shown to be somehow associated with distinct aspects of tumor metastasis such as cell communication protein , motility , adhesion , invasion and tumor immunity associated protein , etc. These results suggest that attaining the ability to metastasize is related to the changed expression of these genes or protein. These data provide insight into the extent of expression differences underlying metastasis-related genes that may prove useful as diagnostic or prognostic markers.Based on all these data, we further uaed RNAi technology by constructing a siRNA expression plasmid to silencing two target genes: B7-1 and XAGE-1b. We used Matrigel simulated cell matrix and nude mice model to test the motion and metastatic capacity after target genes were silenced, and get prefereable results. We can conclude change of B7-1 gene expression influence cell motility. B7-1 partically act as a trigger for NK cells which can lysis tumor cells. Connsidered as member of CTAs family, XAGE-1b is related to cancer metastatic with unknown functions and needs furtherstudy.In conclusion, the present investigation has provide initiate 2-D pattern of adenoidcyatic carcinoma cell lines; has make primary efforts on elucidating molecular mechanism of ACC metastasis; has discovered several RNA or protein molecules involving in ACC metastasis; the possible role of several genes and proteins are disccused. All above has established groundwork for further investigation to clarify the molecular mechanism of ACC metastasis. The intact cloned gene of human XAGE-1b gene and B7-1 gene provide primary possibility to explore the potential role of XAGE-1b and B7-1 genes as a diagonostic or therapeutic marker of Adenooid Cystic Carcinoma clinically.