An Experimental Study About The Role Of Signal Transduction Pathway In Epileptogenesis

Objective To investigate the properties of primary cultured hippocampal neurons from newborn rats in vitro and observe the features of epileptiform discharges induced by Mg²⁺-free media in the cultures, and so to establish a hippocampal neuron epilepsy model for further study on epileptogenesis.Methods After isolated from the newborn rats aged less than 24 hours, the hippocampal neurons were digested and seeded in sterile flasks, 1h later, the cultures were transferred and re-seeded on cover glasses, which had been coated with poly-L-lysine and put in plates in advance. The neurons in cultures were identified by immunocytochemistry with polyclonal antibody to neuron specific enolase (NSE).