Detection Of Human Papillomavirus In Chinese Esophageal Cancer Samples And Studies On Mechanism Of HPV16 E7

The malignant tumor is one of the most serious diseases that endangering people's health. After years of hard work, the scientists have achieved a great number of breakthroughs in the basic research of carcinogenesis, while the exact reasons and mechanism of tumors are not completely known. But it is sure that the development of tumor is a very complex biological process with multifactor and many genes involved. And the virus infection as one of the most important factors among these is attracting more and more attention upon it. Human Papillomavirus (HPV) is a good example, which is believed to relate with many kinds of human tumors. After HPV was confirmed as the definite cause for cervical cancer, the prevention and therapy of cervical cancer were highly pushed forward. So the cervical cancer would possibly become the first tumor that could be controlled completely by vaccination.Besides cervical cancer, the HPV infection also related with head and neck cancer, esophageal cancer and so on. If the relationship between HPV and other tumors was confirmed, the application of HPV vaccine will be expanded. While the incidence of esophageal cancer in China is high, it would be beneficial to study the relationship between HPV and Chinese esophageal cancers further though it has been a controversial question for long time.To investigate the putative role of human papillomavirus (HPV) infection in the carcinogenesis of esophageal cancers in China, we studied the infection rate of HPV in 78 esophageal cancer cases from North China. Among them 48 cases were from high-incidence regions (Anyang city and Shanxi province) and 30 from non-high incidence region (Beijing). And the 23 cancers from Shanxi had the corresponded paracancer epithelium as normal control. All the samples were detected for the existence of HPV 16 DNA by PCR, mRNA in situ hybridization (ISH) and immunohistochemistry (IHC) targeting HPV16 E6 gene. As a result, the infection rate varied with different sample sources: Anyang had the highest HPV16 positive rate, with Shanxin in the middle, Beijing the least. From these two high-incidence regions, there...