| Background: Pancreatic cancer incidence dramatically increases, with radical resection as a sole cure, however with poor prognosis. Over the years, radiotherapy receives more attention, thus in conjunction to surgery or chemotherapy, radiotherapy may achieve better clinical results. However, radiotherapy response varies individually, with clinical reports of radiation resistance. Published reports state an existing relationship between radio-sensitivity and gene expression and regulation, but such reports lack coherence and consistency, since the same gene mentioned to be responsible for radiosensitivity has also been reported to have opposite results, and there yet lacks sufficient evidence regarding the study of genetic promotion of radiation in the treatment and relationship to pancreatic cancer.Objective: With the accomplishment of the Human Genome Project (HGP), substantial genetic data along with increased efficiency in information retrieval using biochip techniques allows for considerable operation and appraisal. Through in vitro radiobiology experiments of pancreatic cancer cell lines, radio-sensitivity comparison may be achieved by using radiobiological parameters, understanding growth alteration of cancer cells after radiation, as well as investigate the mechanism of radiation resistance. Our research objective through laboratory research was to provide experimental evidence for clinical pancreatic cancer radiotherapy. Methods:1. Simulating clinical radiotherapy, pancreatic cell lines SW1990, Capan-1(Cap), AsPC-1(ASPC), P3, PANC-1(Pan-1) and MIAPaCa-2(MIA) were repeatedly given individual dosages of X-ray using a linear accelerator to induce radiation resistance, followed by radio-biologic experiments with both the parental and induced cell lines.2. Utilizing U133-type biochip detection of difference in gene expression of pancreatic... |
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