Loss Of Chromosome Microsatellite DNA And Expression Of Tumor Associated Genes In Human Esophageal Cancer Tissues

Esophageal cancer ( EC ) is a common disease of digestive system, our country has the highest incidence and accountsfor more than one half of the total patients in the world. Previous studies have showed that the loss, mutation and abnormal expression of many tumor suppresser genes were involved in the initiation and development of EC and, to some extent, reflected the aggressive behavior, but no gene specially related to EC has been found until now, and the detailed molecular mechanism of EC is not clear. Further elucidating the role of known genes in EC and searching for new EC genes are of great significance in both the basic research and potential clinic application. In this research, 36 paired human EC tissues collected from Anyang tumor hospital were fixed in Formalin for immunohistochemistry and kept in nitrogen frozen for microsatellite analysis and mRNA differential disply( mDD).1. Microsatellite DNA analysis and immunohistochemistry 25 microsatellite DNA markers respectively linked to tumor suppresor genes p53, p16, Rb, DCC, APC, FHIT, PTEN, DNA mismatch repair genes hMSH2, hMLH1 and Mal, SPRR3 genes whose expressions were down-regulated in EC tissues were examined by PCR-silver staining. p53, p16, Rb, PCNA protein expression in the same tissues were detected by immunohischemical stain to explore the relationship between LOH and gene expression. The main results were as follows:(1) Microsatellite DNA abnormalities in EC were common, 69% patients had 6 or more aberrant loci compared with adjacent esophageal epithelial. The high frequency of LOH( > 25%) was found on DlS484(1q21), D3S1255(3p24-25), D9S162(9p21), D9S171(9p21), IFNa(9p21), D13S170(13q14) and D17S261(17pl3); while high frequency(> 20%) of microsatellite instability (MSI) on D1S484(1q21),...