Experimental Study Of Intracoronary Local Gene Transfer With Protein-coated Metallic Stents Preventing Restenosis After Angioplasty

The long-term efficacy of coronary angioplasty is limited by the phenomenon of restenosis. Intracoronary stent can significantly reduce the — rate of restenosis. However acute, subacute thrombosis and restenosis resulting from vascular smooth muscle cell proliferation are still major problems with current metallic stents. In order to approach a new method to prevent restenosis, a crosslinked protein coating was applied to metallic stent. The biocompatibility and thrombogenicity of the protein coating of stent, the feasibility, efficiency and selectivity of adenovirus-mediated reporter gene(Lac Z gene) transfer to local arterial wall by protein-coated metallic stent as well as the effect of local prourokinase gene transfer, using protein-coated metallic stents, on platelet deposition, early thrombosis and smooth muscle cell proliferation after stent implantation was assessed.In the first part of the studies, the purpose is to assess the biocompatibility and thrombogenicity of crosslinked protein coating of the stent. Metallic stent was woven by 316L medical stainless steel. The...