Study On Dialectically Associated Proteins Of Liver-qi Stagnation Syndrome By Differentially Expressed Serum Proteome Analysis

Syndrome, which bases on the theory of Zang and its outer signs in Chinese medicine and instructs the clinical diagnosis and treatment, has been paid attention in studying of traditional Chinese medicine in recent years. As basic syndrome of liver diseases of Chinese medicine is concerned, liver-qi stagnation syndrome is one of common syndromes in clinical practice and highly valuable for profound research. Some researches indicate that liver-qi stagnation syndrome is likely to result from regulating function disorder of high nervous centre induced by negative psychic stress in which pathological changes of mufti-system including nerve, endocrine, circulation, digestion, immunity, feeling and motion etc are involved. As a complicated syndrome, it is not only liver disorder itself but also complex system of multiple Zang-fu and organs of total body are involved in liver-qi stagnation syndrome. However, only part of known substance change is disclosed so far in some aspects, essence of liver-qi stagnation syndrome is far away from uncovering. Objective :Analyzing the serum proteome of the liver-qi stagnation patients and the health control donors by using the two-dimension gel electrophoresis (2DE) and matrix-assisted laser desorption-ionization time of flight mass (MALDI-TOF-MS), to search the differentially expressed proteins of liver-qi stagnation syndrome for diagnosis.