The Research Of Lupus-like Mouse Model Estabilised By Sm Mimotopes And Interfered By CRP

Background:Systemic lupus erythematosus is a refractory and fatal disease. It is very important to creat mouse model with lupus-like sydrome. Many reseachers had tried to establish lupus-like mice models. But none of the model was satisfmg. Among the researches, peptide induced mice models were highlighted because of its strong specificity. For further investigation, we firstly use Sm mimotope identified by screening a 12-mer random peptide library, which was tridimensional and could induce special immunoreaction that could simulation the reaction between antibody and antigen in SLE patients.Glucocorticosteroid and cyclophosphamide are standard treatments for SLE, while this type of nonspecific immunosuppression is associated with a variety of serious side effects. It is very important to find a novel treatment for SLE. CRP is a normal human serum protein and is not likely to have inherent toxicity or general immunosuppressive activity. Some research found that CRP can be used to treat SLE. Our work is to investigate the functions of Sm antigen and its epitopes expansion in the pathogenesis of SLE, and the possible effect of CRP in SLE treatment.I The isolation and identification of Sm epitopesObjective: To isolate Sm mimotype, a phage peptide library was used.Methods: Sm mimicking epitopes were identified by screening a 12-merrandom peptide library with monoclonal anti-Smith antibody. Threerounds of biopanning were performed and then Dot-ELISA and doubleantibody sandwich ELISA were used to confirm the specific binding ofphages to anti-Sm antibody. Five randomly selected peptides weresequenced.Results: After three rounds of selection, phage clones were amplified. Allthe five peptides selected were found to be positive and shared fivedifferent amino acid sequences. The frequency of IR/SQ/PP was higher.