Local Plasmid-mediated Human Inducible Nitric Oxide Synthase Gene Transfer With Protein-Coated Metallic Stents Inhibits Intimal Hyperplasia After Coronay Angioplasty In Mini Swine Model

Local Plasmid-Mediated Human Inducible Nitric Oxide Synthase Gene Transfer with Protein-Coated Metallic Stents Inhibits Intimal Hyperplasia after Coronary Angioplasty in Mini-Swine Model Background:Nearly a decade has elapsed since the first description of the site-directed transfer of exogenous genetic material into the vascular system. Gene therapy for prevention of restenosis can not be used clinically. The major challenges at present appear to include (1) The availability of vectors that maximize efficiency while minimizing undesirable host responses; (2) The evolution of clinically practical delivery methods that meet the kinetic requirements of achieving efficient gene transfec-tion; (3)the high efficient gene has been searched for the restenosis. Plasmid is safe for the target cell, but has low transfection levels. It would not be expected to be adequate for the molecules that are active only when they are overexpressed in the cells. However, plasmid -based gene transfer has been accompanied by remarkable biologic effects when used to mediate in vivo expression of potent secreted molecule, such as VEGF, so that the effects of gene transfer would be amplified effectively by a paracrine mechanism. For vector delivery, most studies of catheter-based gene transfer have revealed low efficiency except in the context of prolonged vessel occlusion or with ligated branches. "Ideal delivery" is the achievement of deep and symmetric permeation of arterial tissue by vectors. The results of our...