Theory Probing And Experiment Study Of The Correlativity Between Liver And Aging

Object: Probing the close relationship between liver and aging under the direction of TCM theory , then through experiment study , to confirm the mechanism of the correlativity , so as to offer warranty of theory and experiment for postpone aging from liver .The text was divided into two parts: theory probing and experiment study .Theory probing: liver and aging have close relationship through the study of ancient words. The liver called general organ governs the normal flow of Qi and stores blood. 1. The character of liver is active, it connects the congenital and the acquired, so liver is the important factor at the beginning of aging. 2. The liver is responsible for the smoothly ascending and spreading movements of Qi in the body. The dysfunction of the liver would lead to the obstraction of the movement of Qi, the blood stasis andthe phlegm may occur. These are internal pathogenic factors, could cause rhe pathological aging. 3. The temper of the liver is impetuous and firmly, it used to infect other organs, such as kidney, spleen, lung and heart. The liver is called "a black sheep" because of it. 4. The liver regulates the mood. The mental activities of human beings depend on theunobstructed movement of Qi and blood, especial Qi. Abnormal mood cause the movement of Qi unbalance. Many disease are based on disobey of Qi. Sometimes uncontroled mood brings the irregulate of the liver, sometimes disadjust mood due to the dysfunction of the liver.Experiment studyMethods: The mice were randomly divided into five groups: normal control group; model group; Vitamin E-C group; regulating liver low dose group and regulating liver large dose group.The aging rat model was induced by subcutaneous injection of 2%D-Galactose on the back near neck 100mg/kg/d for four weeks and others by 0.9% sodium chloride. Then feed the Vitamin E-C group Vitamin E-C 1.5g/kg.d ,feed the regulating liver low dose group 1.25g/kg.d herd medcined and the regulating liver large dose group 3.75g/kg/d ,the other 0.9% sodium chloride for 4 weeks. We observed MDA, SOD, ANP, CGMP, apoptosis of hepatic cell and ultrastructure changes.Results: 1. Compared with the indexes of the regulating liver groups and Vitamin E-C group and normal control group, modle group revealed the level of SOD in liver tissue (171.6267±18.0425,174.1233±19.3208, 176.748±29.0893,180.464±29.9301 vs 141.523±23.5483,p<0.01,p<0.05 )reduced and the level of MDA in liver tissue (6.3825±0.7285, 6.8558±0.3878, 6.567±0.7894,7.017±0.5345 vs 8.894±0.6133,P<0.01) increased. 2. regulating liver groups all can decreased obviously in serum ANP(133.3281±80.1879, 147.1493±71.7597 vs 559.2754±116.1705, 575.2104±319.192, 380.2496+358.8657, P (0.01, P (0.05) .And can increased the level of serum CGRP (221.9872±44.1605, 214.8828+116.2725 vs 172.149±43.7388, 158.7331±52.0614, 143.291±25.7986,P<0.01). 3. There were obviously apoptosis in model group; regulating liver can decrease the rate of hetepic apoptosis(26.92±3.18,30.07+3.43 vs 36.71±2.31,P<0.01) .there is significant different between high dose regulating liver group and low dose regulating liver group(26.92±3.18 vs 30.07±3.43,P<0.05) 4. Regulating liver can obviously improve the ultrastructure pathological changes made by D-Galactose.Conclusion: There were many changes in model group, such as: the decrease of SOD, the increase of MDA, ultrastructure pathological changes etc. It means the aging model was successfully founded. Regulating liver therapy can decrease the level of free radical, restrain apoptosis, improve blood circulation, though these it can postpone aging.