The Effects Of Rhodiola Rosea L.on Lung Injury Of Rat Induced By Bleomycin

Purpose: According to Traditional Chinese Medicine theory and the effect of RRL on SARS in prevalent period, to study the effect of RRL on rat lung injury induced by BLM on 3 day. 7 day and 28 day in terms of the lung function, lung pathology and TNF- a , GM-CSF> MCP-1 in rat serum, To analyze the treatment mechanism of RRL on inflammatory and fibrosis in lung injury.Method: The lung injury modal is made by intratracheal bleomycin (5mg/kg) and the rats are divided into control group, BLM group, BLM+RRL group and BLM+MPD group. Compute Tomograph and blood gas analyzer are employed to indicate lung function and image changes. The lung coefficient and lung perfusion index are applied to demonstrate the inflammatory edema and perfusion degree of lung. H.E. staining, V.G. staining and transmission electron microscopy (TEM) are used to illustrate lung pathology feature. The immunohistochemistre stain for GM-CSF. a -SMA in lung. TNF- a , GM-CSF. MCP-1 in serum are checked with enzyme linked immunoabsorb assay. The collagen content in lung is analyzed by hydroxyproline measurement. Result: CT illustrates the pieces of cloudlike shadow on 3day in BLM group. Some of them are bigger than others and the boundary is not clear, on other hand, the density come to be thinner from the center of lung gradually, on 28day,the CT show the high-density knot or netlike shadow distributed from lung center to border region, meanwhile, lung interstitial becomes deformed companied by over expended bronchus. The image changes of RRL+BLM group are lighter than BLM group on 3day and 7day,but similar on 28day except a little less density.Although the lung coefficient and lung perfusion index are increase at three different points in BLM group, RRL+BLM group and MPD+BLM group, especially on 3 and 7day. RRL+BLM group is lower than BLM group, but PaO2 higher on 3and and 7day.On 3day and 7day after BLM administration, the pathology study illustrates acute pulmonary alveoli and acute interstitial inflammation, showing extensive infiltration of white cells and widespread edema and hemorrhage. Meanwhile, TEM manifests type I pulmonary epithelium transforming, broken and detachment; but type II cell inproliferation and swollen. The alveoli contain many lamina-body and endothelium is damaged. The RRL+BLM group much lighter than BLM group. The lung interstitial is more abnormal obviously on 28day under light microscopy, including thickening of bronchi wall, proliferation of smooth muscle cell, lymphocyte accumulation around, widening of alveolar partition and increase of fibroblast. TEM demonstrates proliferation of fibroblast, accumulation of collagen in lung, and disorder of the structural systems of microcirculation and alveoli in BLM group. The characteristics of RRL+BLM group are lighter than BLM group.Immunohistochmical staining shows the GM-CSF isn't expressed or weakly expressed in lung of control group. Controversially, it is over expressed in three groups on 3day and 7day after BLM administration, although the positive degree of RRL+BLM and MPD+BLM groups is lower than BLM group. But they are not distinct among three groups on 28day. The ELISA evidences that TNF- a . MCP-1. GM-CSF in serum of three groups are raised on 3 and 7day after BLM administration. The level of RRL+BLM lower than BLM group, but they are not different apparently on 28day. The content of collagen of three groups are more than control and there are not evident distinct among themselves on 28day. Conclusion:l.The lung injury induced by BLM includes inflammatory reaction and fibrosis process in whole course. The injury in early stage mainly shows acute inflammatory leakage and hemorrhage, which same as SARS in clinic in early stage. Otherwise, the lung injury mainly produces chronic inflammation and fibrosis in late stage. 2.Rhodiola rosea L(RRL) have the ability to suppress edema, leakage and hemorrhage caused by inflammation through disturbing synthesis of TNF-a , MCP-K GM-CSF in early stage. In addition, RRL is capable of protection endothelium from BLM damage...