The Study Of Constructing The Adenovirus Vectors Expressing The Soluble Ectodomain Of TGF-βRⅡ And RANTES Gene And Then Combined Using In Anti-Cancer Experiments

Projects: To analyzed the mechanisms and valuated the anti-tumor effects of the RANTES gene and soluble extracellular domain of TB RII that mediated by adenovirus vector. Methods: Cloned the human and mouse origin RANTES gene and soluble extracellular domain of T B RII by RT-PCR method and constructed the adenovirus vector using the AD-easy XL Adenovirus Vector System. Using the chemotactic test and ELISA to test the functions of the RANTES gene and soluble extracellular domain of TB RII which mediate by virus. And valuated the effects on immune cell in vitro and the anti-cancer function in vivo. Results: RANTES gene and soluble extracellular domain of TB RII could be cloned by RT-PCR pathway and be constructed into adenovirus vector using AdEasy XL Adenoviral Vector System kid. Functional tests showed that RANTES mediated by adenovirus vector could mimic the natural chemotactic activity in vivo or in vitro and the soluble extracellular domain of T B RII could counteract the TGF-B signaling in vitro. When used RANTES gene and soluble extracellular domain of T BRII that mediated by adenovirus vector to treat the H22 bearing mice with ascites combined, encouraging outcome was seen that the volume and increasing rate of ascites, as well as the survival time are distinctively different from the controls. Conclusion: Combined using RANTES gene and soluble extracellular domain of TBR II that mediated by adenovirus vector could improve the anti-cancer immune response and inhibit the progression of the cancer. It gives a new way to treat the cancer.