Empirical Study On Mechanism Of Action About Calcium Metabolism Of The Bone, And Regulation Of The Nanometer Traditional Chinese Medicine Complex To Osteoporosis

Purpose: In order to approaching the patho of the osteoporosis or the regulatory mechanism of the nanometer Calcium and the traditional Chinese medicine,observing the effects of the nanometer Calcium and the nanometer Calcium-traditional Chinese medicine complex prescription in Calcium absorptory and deposition,we observed the each indicatrix of the bone calcium metabolism and the effects of the BGP and BMP-2 from the gene and proteinum express level to the osteoporosis rat due to Kidney-Deficiency.Method:Using the fluidized-bed opposed jet mill and the nano technology,we prepare the nanometer Chinese traditional medicine.Using the method of ovariectomy, we established the rat model of osteoporosis due to kidney-deficiency.Using dual energy X-ray bone density instrument XR-36 model to detect the bone mineral density of the left femur of the rats. Using the strain test system, we examined the biomechanics index according the tension and three-spot bending test; we also examined the bone calcium of the lumbar vertebra through plasma emission spectroscopy . We examined the serum calcium and magnesium by using the nomal biomechanics method,and examined the serum acid phosphatase and osteocalcin by using the Elisa method. We also examined the expression of BGP and BMP-2 gene with the method of RT-PCR and the proteinum expression with the method of WESTERN-BLOT in the bone tissue of osteoporosis rats due to Kidney-Deficiency.Result:When the rats had been removed the ovaries,their weights of body raised and their weights of uterus depressed obviously.All of their bone density,calcium content of bone,serum calcium and magnesium ionic concentration were depressed obviously.Otherwise,the content of the serum osteocalcin was depressed and the TRAP increased obviously.The biomechanics index and property of the bone depressed obviously.The BGP and BMP-2 gene and proteinum expressiones were belower than these of the normal group.Through medication,Nano-Ca could increase the femoral bone density of desovary experiment rat obviously,and the effects surpassed these of masculine medicine control group, surpassed these of Nano-Ca and invigorating the kidney group. But to the head of remur, the Nano-Ca and invigorating the kidney group surpassed Nano-Ca group slightly. Nano-Ca could increase calcium content of bone of lumbar vertebra,serum calcium and magnesium,the effects surpassed these of masculine medicine control group and of Nano-Ca and invigorating the kidney group. Through medication,the biomechanics index and property of the bone were improved or recoveried, and the effects of all groups had no obviously difference.The tretment groups could improve the content of BGP and depress the content of TRAP which in the blood serum, but both of the tretment groups and control groups failed to attent the normal value. The Nano-Ca group and the Nano-Ca and invigorating the kidney group could promote the BGP and BMP-2 gene and proteinum expressiones obviously. The Nano-Ca groupexpressed most strongly, and the Nano-Ca and invigorating the kidney group expressed the next, furthermore the expressing were dose dependent.Conclusion:When the single traditional Chinese medicine was zoomed to nanometer.the absorption and deposition of the medicine were improved obviously.The effects of treatment were improved too.The machines maybe were that the Ca-ionic concentration in microcirculation was affected,and the BGP and BMP-2 gene and proteinum expressiones in exterior and interior of osteocyte were influenced,thus the deposition of the Ca and the mineralization of the bone were improved,the bone resorption was refrained,and the bone formation was promoted.