The Research Of Rooting Mechanism Of Malus Halliana & Malus Prunifolia Cutting

Under various treatments of exogenous phytohormone (such as IBA,NAA) in different concentration and soaking time, this dissertation tested the variances of some endogenous phytohormone (IAA,ABA,ZR,GA, etc.) and soluble proteins in cuttings with the techniques of ELISA and SDS-PAGE during the different Crab apple. cuttings' rooting process, figured out the relations among their endogenous phytohormones and soluble proteins as well.This paper also researched the rooting process of Crab apple cuttings' in which the inner genetic factors controlled by the way of proteins' conveyance, discovered the kinds of proteins which affected rooting during the cutting process. It also explored the connection that endogenous and exogenous phytohormones affected rooting of Crab apple cuttings.by combining BP. Through more than three years research work, some achievements have been obtained as follow:1.Exogenous phytohormone also effected the rooting rate of cutting. Variance analysis showed that IAA had significant function on cutting. And Gibberellin mixed with IAA showed to promote each other on breeding branch.2.Different date of cutting affected the rooting rate markedly. Withn suitable period, the rooting rate was higher when cutting taken on later date,and the exogenous phytohormone lowered its' effect. 3.The studies showed several factors, ie. water content, soluble protein, soluble sugar, and polyphenol oxidase activity as well affected the rooting rate. According to coefficient analysis, the rooting rate had negative correlation with bound water, and positive with free water. The water content dropped until 9 days after cutting. soluble protein, soluble sugar, and polyphenol oxidase activity improved slowly. The rate of IAA/ABA and IAA/ZR peaked in 4 to 6 days in cuttings, which means that the content of IAA increased quickly at initial period was very important for cell growth and root primordium formation. 4.The structural investigation of cutting showed that: (1) There was no incubate root primordium in cuttings of Malus prunifolia Borkh, The induced root primordium of developed into the adventitious root, and orginated form the division and differentiation of the vascular cambium cell touching with the pith ray. After 15 days, adventitious root primordium developed into young adventitious roots. (2) In the cutting stem of Malus halliana Roehne, The callus generated after 10 day of cutting and not only form much sclerenchyma, but also form adventitous root primordium, and 40 days more later, adventitious root primordium developed into young adventitious roots.5. During the cutting process, for Malus halliana Roehne, the soluble proteins in cut of 46KD, 39KD, 36KD, 28 KD, 26KD, 24KD are related to the rooting. The soluble proteins in 39KD, 26KD, 24KD control the callus growing and adventitious roots differentiating, consequently, they disappeared as the cutting began to generate adventitious roots. The soluble proteins in 28KD and 31KD appeared during the callus and root developing. It seems that the soluble protein in 46KD in cutting was the possible obstacle to the callus differentiation of the cuttings rooting.For Malus prunifolia, 65KD, 52KD, 43KD, 39KD, 31KD, 28KD, 26KD, 22KD are related to its rooting. The soluble proteins in 65KD, 39KD, 26KD, 22KD control adventitious roots primordium generating and adventitious roots differentiating, and they disappeared as the cutting began to generate adventitious roots.