Study On Structure And Bioactivities Of Lycium Barbarum Polysaccharide

As a natural polysaccharide, Lycium barbarum polysaccharide (LBP) plays an important role in the Chinese traditional medicine. In this study, the extraction, structure and bioactivities of LBP were studied. And to further study the LBP's function mechanism, laser scanning confocal microscope, flow cytometer and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) were performed in this paper. What's more, a simple and reliable method was put forward to determine the content of polysaccharide in Lycium barbarum Extract. The main results are as follows:1 Characteristic and structure of LBPA new separate procedure was put forward in this study: Fructus lycii was chopped into pieces and extracted by different solvents, then the crude extract was decolored, removed protein and treated by gel chromatography, after have been vacuum concentrated and freeze-dried, a pure product of LBP was gained. The yield was 2.21%.With GC analysis the monosaccharides in LBP were composed of Rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), galactose (Gal) with the molar ratio of 0.72: 5.82: 0.52: 0.26: 1: 4.48. The content of neutral sugar, galacturnic acid and protein in LBP was 45.09%, 46.76% and 6.98% respectively.According to the IR spectra, it contains -COOH group, -OH group, -NH₂ group, -NH-group, β-D-glucose, α-D-mannose and α-D-galactose. The results of β-elimination reaction indicated that the chain of polysaccharides and protein were connected by O-linked covalent bond. The Congo-red reaction and I₂-KI reaction indicated that no triple helix structure was occurred in LBP molecule with some long side chain and high branch chain. The images got by scanning electron microscope showed that the LBP was collected in fragment shape.2 Determination of Polysaccharide in Lycium barbarum ExtractFor the determination of polysaccharide in Lycium barbarum Extract, a simple and exact method was firstly put forward in this study. After the monosaccharide, oligosaccharides and pigment were removed from Lycium barbarum extract, which...