Effect Of Lactobacillus Plantarum On Diarrhea Induced By Escherichia Coli In Newborn Rabbits

The intestinal microbiota refers to a complex community of microorganisms that reside in the gut.The intestinal microbiota plays a crucial role in the health and immunity of animals,including rabbits.One of the common pathogenic bacteria found in the gut is Escherichia coli,which can cause diseases such as diarrhea and gastroenteritis.Existing research suggests that probiotics play a positive role in maintaining the balance of the intestinal microbiota,offering new avenues for the treatment of E.coli infections.Plantarum is a natural probiotic that can colo nize the gut,maintaining the balance of gut microbiota,improving intestinal health,and alleviating diarrhea.Studying the effect of plantarum colonization on E.coli infection can provide a better understanding of the role of probiotics in maintaining g ut health and preventing gut infections,while also offering new insights for exploring and developing safer and more effective alternative drugs.Methods:The purpose of this experiment was to assess the clinical efficacy of plant lactobacillus in managing colibacillosis-induced diarrhea in juvenile rabbits.Sixteen three-month-old New Zealand white rabbits were randomly allocated into four groups:Control group(1 m L/d saline),Model group(0.3 m L 1×10~9 CFU/m L Escherichia coli solution+0.2 m L/d saline),Low-dose probiotics treatment group(L)(1 m L 1×10~7 CFU/m L/d plant lactobacillus solution),and High-dose probiotics treatment group(H)(1 m L 1×10~8 CFU/m L/d plant lactobacillus solution).The experiment was conducted over a ten-day period,during which the rabbits were observed and recorded daily for any symptoms of diarrhea.The model of colibacillosis-induced diarrhea was established by intraperitoneal injection of 0.3 m L 1×10~9CFU/m L Escherichia coli solution+0.2 m L/d saline for the first three days.After the successful modeling,the Control and Model groups were orally given saline,while the L and H groups were continuously orally treated with live plant lactobacillus solu tion for seven days.The clinical application value of plant-derived lactobacillus colonization on E.coli-induced diarrhea in young rabbits can be evaluated through diarrhea scoring,clinical blood indicators,inflammatory markers,antioxidant markers,and intestinal function indicators.Research findings:(1)Colonization of plant lactobacillus can significantly reduce the diarrhea score and improve the diarrhea condition of young rabbits infected with Escherichia coli.(2)Colonization of plant lactobacillus can significantly increase the height of villi(VH)in the ileum and colon,as well as the VH/V ratio,while reducing the depth of crypts(CD)in the ileum and colon,thereby improving the morphological structure of the intestine.(3)Colonization of plant lactobacillus can reduce the levels of serum IL-1,IL-1β,IL-6,and TNF-α,while increasing the levels of serum IL-4 and IL-10,thereby alleviating the inflammatory response in young rabbits.(4)Colonization of plant lactobacillus can significantly reduce the levels of MDA,CRF,and GSH in the serum,increase the level of SOD,and enhance the antioxidant capacity of young rabbits.(5)Colonization of plant lactobacillus can reduce the levels of serum DAO and D-lactic acid in young rabbits,thus alleviating the damage to intestinal function.Conclusion:The colonization of Plantarum Lactobacillus has proven to be effective in ameliorating colibacillosis-induced diarrhea in young rabbits.Moreover,it has shown to improve the intestinal morphology,exhibit anti-inflammatory and antioxidant properties,and alleviate intestinal functional impairments.