Genetic Diversity Analysis And Regional Applicability Evaluation Of Camellia Oleifera Varieties In Jiangxi Province

Camellia Oleifera is one of the most important woody oil plant species in China,which is called"the four largest woody oil plants in the world".Excellent varieties are the key to the development of Camellia industry.At present,although the main push improved varieties have been confirmed in all provinces,the habitat of different regions in the province is quite different,and the distribution pattern of varieties in different regions is not clear yet.Based on SSRseq technique,the genetic diversity of 25 Camellia varieties in Jiangxi Province was analyzed,and the growth difference,stability and adaptability of Changlin varieties in different regions of Jiangxi Province were evaluated,some suggestions on the allocation of varieties in different regions were put forward.The main findings are as follows:(1)Based on SSRseq technique,the products were genotyped by multiplex PCR and 15 pairs of SSR primers were screened.A total of 142 Alleles were detected.The effective alleles ranged from 2.34 to 11.86,with an average value of 5.38.The observed heterozygosity ranged from 0.493 to 0.925,25 Camellia varieties had inbreeding to some extent,the average inbreeding coefficient was 0.02,the PIC value ranged from 0.57 to 0.91,the average value was 0.76,and C_O74 primer was the best.The genetic distance ranged from 0.27 to 0.48,with an average value of 0.37,and the genetic distance was small.25Camellia varieties had close genetic relationship,GF20 and G8 were the most distant,CL4and CL18 were the closest,25 varieties of Camellia were classified into 5 categories by cluster analysis.(2)The growth and fruiting of Camellia had significant difference among different varieties and experimental sites,and had significant correlation with geographical location and environmental factors.From south to north,the body of Camellia is gradually tall,the yield is gradually increased but the economic index of fruit is gradually worse.Luxi County tree body is significantly smaller,but the fruit economic index performance is best,CL3,CL53,CL18 performance is superior,the specific leaf area is significantly higher,the adaptability to the environment is stronger,Yifeng tree body is significantly higher and the fruit is bigger,xiajiang County fruit was smaller,Shangrao Leaf was larger,Xiajiang County leaf was smaller,and Jinxi County Leaf shape index was significantly higher.The yield per plant was lower in the younger Luxi County experimental site than that in the Shangrao experimental site of the same age and was significantly lower in all the experimental sites,but the yield per projected area was higher and the production potential was higher.The yield per plant and the yield per projection area in Jinxi County and Poyang were better.(3)CL3 plant height and dry seed rate were significantly higher,CL4 crown width,seed kernel oil content and fresh fruit oil content were significantly higher,CL18 leaf length,leaf circumference and leaf area were significantly higher,plant height and fruit shape index were significantly lower,fruit shape index was less than 1;The height of CL40trees,the rate of kernel,the leaf length,leaf circumference and leaf area of CL53 were significantly lower than those of CL53,and the transverse longitudinal longitude,single fruit weight,single fruit volume and fresh seed rate were significantly higher than those of CL53 The yield per plant and the yield per projected area of CL53 were better than that of CL18.(4)The Variation Coefficient of tree body,leaf and fruit growth index was 10.66%～40.63%,the variation coefficient of crown width was 40.63%,the variation coefficient of kernel oil content was 10.66%and the repeatability was 0.53～0.99,the oil content repeatability(0.53)of fresh fruit was the smallest,and the fruit diameter repeatability(0.99)was the largest.(5)Gge biplot was used to analyze the fertility and stability of Camellia cultivars in Changlin varieties,and the representativeness and differentiation of the test site.The results showed that the yield of fresh fruit per plant and the yield of oil per plant of CL3,CL40and CL53 were high,but CL40 was not stable,and the yield of projection area of CL53and CL3 were high and stable;when the evaluation index was the yield per plant,the representativeness and differentiation of Shangrao and Jinxi County were better;Luxi and Jinxi County have good representativeness and differentiation,so Jinxi County can be used as a test site for variety evaluation and selection.(6)The yield per plant of CL53and CL3 was stable and high-yielding,which was suitable for popularization in the whole province,and CL18 performed well in the north and west of Jiangxi,but the overall performance of low yield and poor stability,suitable for these two areas as a small number of varieties planted.