| Fluorene ring polyelectrolytes,as a luminescent material,have shown high sensing performance due to their "molecular line" effect.In recent years,they have been used as fluorescence probes to detect a variety of small molecules,and have been widely used in the fields of materials,biology,medicine,and chemical industry analysis.In this study,one of the ionic polymer(IP)was selected as the fluorescence probe to detect the small molecules of four aromatic derivatives,namely ellagic acid(EA),tartrazine(TTZ),rutin and oxytetracycline(OTC).EA was identified and detected by IP as the fluorescence probe.EA reduced the fluorescence of IP in several selected solvents,and the fluorescence response reached a stable value in an instant.The quenching efficiency of IP was 80.09%-92.58%,and there was a good linear relationship with the EA concentration in a certain range,and can realize rapid and sensitive identification.The limit of detection reached the level of 1.2989 mol / L-1.8979 mol / L micromolar detection.In contrast,such as urea,saccharides,vitamins,anions and cations,amino acids,antibiotics and other coexisting substances only quenched the fluorescence of IP by-18.85%-20.85%,indicating that the probe has good selectivity for EA.At the same time,IP also showed excellent anti-interference ability for EA recognition,because when the above substances were present,the one that has the greatest effect on IP fluorescence was only 5.83%.The recovery experiment was complicated by IP in the actual sample of commercial make-up water spray containg a certain concentration of EA.The recovery rate was 98.06%-109.36% and the relative standard deviation was 1.41%-2.41%,demonstrating that the method had high precision and good repeatability.TTZ was identified using IP as the fluorescent probe.TTZ could quench the fluorescence of IP by 81.88%-97.79% in the selected solvent system,and the quenching efficiency showed a good linear relationship with TTZ of 0-10 mol / L,and the detection limit could achieve at the micro-molar trace level of 1.0523 mol / L-1.4712 mol / L.The urea,sugars,vitamins,cations and anions,amino acids and food additives potentially exising in the actual complex environment,which made the fluorescence change of IP only within the range of 14.54%-16.81%,far less than the high selectivity of the probe for TTZ.Moreover,when these interfering substances existed,the recognition of TTZ by IP would not be greatly interfered.In addition,By used this identification method,the recovery rate of actual samples in commercial soft drinks was 97.48%-106.50%,and the relative standard deviation was 1.07%-2.51%,demonstrating the good accuracy and repeatability of this method.IP was used as the fluorescent probe for the specific recognition of rutin.Rutin in the selected solvent could reduce the fluorescence of IP,with the quenching efficiency in the range of 74.52%-90.80%,The reaction of the two could achieve instantaneous equilibrium so IP performed the high efficiency and rapid detection of rutin.The fluorescence quenching efficiency of IP exhibited a good linear relationship with the concentration of rutin in the range of 0-16 mol / L,and it realized sensitive recognition,and the limit of detection could be as low as 1.5956 mol / L.The complex environment might contain urea,saccharides,vitamins,anions and cations,amino acids and antibiotics,all of which only affected the fluorescence of IP by-15.71%-10.29%,indicating that the probe has good selectivity for rutin.Besides,the existence of interfereding substances would not affect the identification of rutin by IP,and show excellent anti-interference ability.The experimental results of spiked recovery of the actual sample solution of healthy student urine after adding rutin showed that the recovery rates were 97.00%-113.00% and the relative standard deviation was 1.48%-2.88%,demonstrating that IP had high accuracy and good repeatability for the quantitative detection of rutin.The identification of OTC using IP as the fluorescent probe was studied.OTC quenched the fluorescence intensity of IP to 81.51% to 94.23% in the selected solvents,providing a transient,and stable fluorescence response.The fluorescence quenching efficiency of IP showed a good linear relationship with the OTC concentration in the range of 0-20 mol / L,and the limit of detection could be as low as 1.1686 mol / L-2.1338 mol / L.After urea,saccharides,vitamins,anions and cations,amino acids and antibiotic interfering substances were additionally added,the above fluorescence recognition effects were not significantly affected.The spiked recovery experiments were performed on the actual sample solution of healthy student urine after adding OTC,and the recovery rates reached 99.75%-111.42%,and the relative standard deviation was 1.64%-2.98%,impling that the method had high precision and good repeatability.The fluorescence emission spectra of IP and the UV-Vis absorption spectra of these four small molecular aromatic derivatives were partially overlapped,and fluorescence resonance energy transfer(FRET)and internal filtration effect(IFE)occurred between them.Besides,the fluorescence anisotropy was increased after the interaction of the two.All four aromatic derivatives could form larger aggregates with IP,which was more conducive to the generation of π-π stacking,hydrogen bond interaction and electron transfer.In addition,under the static quenching mechanism,it led to the occurrence of IP fluorescence quenching. |
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