Screening Of Coronavirus-host Cell Interaction Genes

Coronavirus is one of virus for Nidovirales,which is a lethal zoonosis with high pathogenicity and have a longest RNA genome among all of viruses in the world up to now.Among them,SARS-Co V and MERS-Co V are currently coronaviruses that pose a serious threat to human health,making research on such viruses urgent.But due to the harmful nature of viruses,these viruses cannot be directly studied.Mouse Hepatitis Virus(MHV)is a model virus in coronavirus research.It is a well-studied coronavirus at the cellular and animal levels and is used as a safe model for studying Co V.The model can be helpful for understanding other coronaviruses and human coronaviruses.In this experiment,MHV-infected mouse-derived dendritic cells(BMDC)were used to infect different viruses(MHV,VSV,HSV-1).Simulating an acute infection by the virus in cell and detecting the production of various cytokines,such as interferon or other cytokines.Then analyzed by transcriptome sequencing and proteome sequencing to obtain differentially expressed genes/proteins in the cells at difffrent infection conditions.Using GO gene annotation and KEGG pathway enrichment analysis,every gene/protein is classified according to the biological processes,molecular function,and cellular components.Then selecte genes/proteins that play an important role in each pathway and reduce the expression of the gene by RNA interference to find the relationship between those genes and virus replication.Finding out the key factors that may play a role in cells after acute infection and initially verify its function,providing new ideas for the future research of mechanism in anti-virus.