| In this experiment,20 small-tailed Han sheep rams with a similar weight(34.4±1.38kg)were selected and used for a single factor completely randomized trial design.They were divided into 2 groups with 2 replicates in each group and 5replicates in each group.The control group was fed basal ration,and the test group was fed basal ration + Allium Mongolicum Regel volatile oil(addition amount was calculated based on in vitro experiments).The trial Period is 75 days,of which the Pre-trial Period is 15 days and the trial Period is 60 days.During the feeding Period,the leftover feed was collected and weighed every day to calculate the average daily feed intake.On the 1st,15 th,30th,45 th and 60 th day of the normal feeding Period,the food was weighed on an empty stomach before morning feeding and recorded.The test data was used to calculate the daily gain and feed-to-meat ratio;one week before the end of the test,samples of sheep’s feces were collected continuously to determine the apparent digestibility of nutrients;after the end of the feeding test,each treatment group randomly selected 6 A total of 12 sheep were slaughtered.After slaughter,the duodenum,jejunum and ileum tissues were quickly separated,and 2 cm was taken from each intestinal segment to determine the changes in the structure of the intestine,the length of the villi and the secretion.Crypt depth and calculate the V/C value.The results showed that:(1)From day 0 to day 45 of the experiment,there was no significant difference in the average daily feed intake of the two groups of sheep(P>0.05).From day 45 to60,the average daily food intake of the experimental group was significantly higher than that of the control group.(P<0.05);The average daily gain of the two groups was not significantly different from the 0th to 30 th day of the experiment(P>0.05),and the average daily gain of the experimental group was significantly higher than that of the control from the 30 th to 60 th day of the experiment group(P<0.05);the average feed-to-meat ratio between the control group and the test group was not significantly different(P>0.05)on the 0th to 15 th,15th to 30 th,and 45 th to 60 th days of the experiment(P>0.05),and the 30 th day of the experiment The average feed-to-meat ratio of the control group was significantly higher than that of the test group at ～45days(P<0.05),and from the 30 th day of the test,the feed-to-meat ratio of the control group was higher than that of the test group.(2)Allium Mongolicum Regel essential oil can increase the villus height of duodenum,jejunum and ileum of meat sheep,but the difference is not significant(P>0.05);Allium Mongolicum Regel essential oil can reduce the crypt depth of duodenum,jejunum and ileum of meat sheep.The difference is extremely significant(P<0.01);Shallot essential oil can increase the V/C value of duodenum,jejunum and ileum of mutton sheep,and the difference is extremely significant(P<0.01).(3)Allium Mongolicum Regel essential oil can improve the digestibility of dry matter,organic matter,Protein,neutral detergent fiber,calcium and phosphorus in mutton sheep.Among them,the digestibility of dry matter and Protein is significantly different(P<0.05),while the digestibility of acid detergent fiber compared with the control group,the fat digestibility decreased,and the difference was not significant(P>0.05).Shallot essential oil can effectively improve the growth Performance of mutton sheep and the apparent digestibility of nutrients,and significantly improve the morphological structure of the small intestine of mutton sheep. |
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