Study On The Effect Of Tea Polyphenols Disinfection Methods On Drinking Water Resistance Genes

With the improvement of people’s living standards,many people have begun to pay attention to an emerging environmental pollutant-antibiotic resistance gene,in addition to the conventional drinking water quality testing indicators.Traditional drinking water disinfection methods have little effect on resistance genes,so a disinfection method that can effectively inhibit bacteria and have a strong removal effect on resistance genes is urgently needed.As a green and natural disinfectant,tea polyphenols have strong oxidative properties and broadspectrum antibacterial properties.At the same time,they can destroy the molecular mechanism of resistance genes and affect the transfer and spread of resistance genes.Tea polyphenols can be used in combination with ozone and ultraviolet rays.This article mainly studies the effect of tea polyphenols and ozone-tea polyphenols combined disinfection method and ultraviolet-tea polyphenols combined disinfection method on the resistance genes,and at the same time explores the influence of the changes in the microbial community on the resistance genes.When tea polyphenol is used as a disinfectant alone,when the dosage is 200mg/L,it can effectively inhibit the growth of bacteria.However,the 200mg/L concentration of tea polyphenols cannot meet the requirements for color in the Sanitary Standards for Drinking Water(GB5749-2006),so tea polyphenols are more suitable for use as auxiliary disinfectants.Studies have shown that low concentrations of tea polyphenols have a strong reduction effect on resistance genes,but when the concentration of tea polyphenols exceeds 200 mg/L,the reduction effect is weakened.200mg/L of tea polyphenols has a poor effect on the overall removal of vancomycin resistance genes;it can effectively inhibit the spread of tetracycline resistance genes,which can reduce the overall abundance of tetracycline resistance genes;the control effect of resistance gene like penicillin and penicillin resistance gene is better.The role of tea polyphenols against resistance genes mainly includes two aspects: the way of inhibiting the spread of resistance genes and the molecular mechanism of destroying resistance genes.Both the ozone-tea polyphenol combined disinfection method and the ultraviolet-tea polyphenol combined disinfection method can control the resistance genes in drinking water,and the control effects of different methods are different.The combined disinfection method of ozone and tea polyphenols has a good control effect on β-lactamases,tetracyclines,and aminoglycoside resistance genes.The combined disinfection method of ultraviolet rays and tea polyphenols has a better control effect on β-lactamase resistance genes.When drinking water contains a certain concentration of a certain resistance gene,it can be selected based on the effects of the two combined disinfection methods.When the type and concentration of resistance genes contained in the source water are unknown,the ozone-tea polyphenol combined method is preferred as the disinfection process,which has the advantages of wider coverage,better removal effect and stable effect than the combined ultraviolet-tea polyphenol process.The composition of the microbial community in the water samples after the combined disinfection process of different tea polyphenols is roughly the same,but the proportions are slightly different.The ozone-tea polyphenol combined disinfection process has a more prominent effect on the reduction of microbial diversity than the ultraviolet-tea polyphenol combined disinfection process.After the combined disinfection process of ozone-tea polyphenols and ultraviolet-tea polyphenols,the abundance of genes with specific functions of microorganisms changes greatly.The histogram of the KEGG pathway reflects that the two combined disinfection methods have a significant reduction in the abundance of genes that regulate the folding,classification and degradation of genetic information,replication and repair,and transcription and translation.However,the ozone-tea polyphenol combined disinfection method has a better reduction effect than the ultraviolet-tea polyphenol combined disinfection method,which also explains the reason why the ozone-tea polyphenol combined disinfection has a better effect on resistance gene reduction.