Click-derived Fluorescence Chemosensors For Phosphorylated Anions Recognition

Phosphate anion plays an important role in organisms, environment, industry and and other fields. For example, phosphates have been widely used in food processing, detergent, the paper industry, fertilizer, etc; Too much phosphorus in water can lead to eutrophication in inland waterways; The abnormal phosphorylation of the human body can also reflect the human body the appearance of a number of diseases. Therefore, detection of phosphate anion has become a more concerned direction. Among all test methods, the fluorescent sensors get the favour of people, because of its simple, convenient, quick and sensitively.In this paper, a series of fluorescent sensors containing triazole/triazolium had been synthesized, and through the absorption, emission spectrum, NMR and DFT calculation etc, its selective recognition ability of anion is studied.1. A series of compounds Fc-1a,Fc-1b and Fc-1c based on ferrocene and triazole with different fluorescence groups were designed and synthesized and their anion sensing properties were studied. In acetonitrile solution, by electrochemical experiments, fluorescence spectrum experiment to achieve the double signal recognition of H2PO4?. And the compound Fc-1a,Fc-1b and Fc-1c and H2PO4? complex which is a rotating mechanism were studied by the method of Job curve, 1HNMR titration and DFT calculations. In fluorescence spectrum experiments, we found that the complex process of compounds and H2PO4? was controlled by kinetic, so the kinetic influence was further studied to compound Fc-1a,Fc-1b and Fc-1c recognized H2PO4?.2. A novel pyrene- functionalized polynorbornene(P3-1) bearing sulfonamide NH and triazolium donors has been synthesized for ratiometric fluorescence sensing of PPi and in aqueous solution. Inaddition, P3-1 is also used to monitor intracellular PPi and to detect PPi released during polymerase chain reaction3. A novel polynorbornene fluorescent sensor(P4-1) has been synthesized by the ring opening polymerization(ROMP) with amide group NH and triazolium(C H)+ as binding sites and anthracene as fluorescent signal groups. In aqueous solution, P4-1 coud selective recognize ATP by nonmetal fluorescence sense. Compared with other anion, P4-1 showed the specific fluorescence quenching for ATP, and the detection limit was 25 n M. The sensor has the potential of biological applications.