| Objective:To analyze the infection situation of human rhinovirus(HRV)and enterovirus(HEV)in hospitalized children with respiratory tract infection,so as to provide basis for its prevention and treatment and vaccine research and development.Methods: From January 2017 to December 2017,386 samples of pharynx swabs from children hospitalized for respiratory tract infection in the People’s Hospital of the Xinjiang autonomous region were collected,the general information and clinical data were also collected.The VP4/VP2 gene fragment of HRV/HEV was amplified by reverse transcriptase polymerase chain reaction(RT-PCR),and some positive samples were selected for sequencing and phylogenetic analysis.Clinical correlation analysis was carried out with SPSS17.0.Results: The positive rate of HRV/HEV in pharynx swabs samples of 386 children with respiratory tract infection was 20.21%(78/386).Among them,HRV positive was found in 19 cases,HEV positive in 50 cases,and HEV and HRV mixed infection in 9 cases.The detection rates of HRV/HEV in boys and girls were 17.45%(41/235)and 24.50%(37/151)respectively,and the difference between the two groups was not statistically significant(χ2=2.839,P=0.092).The detection rates of HRV/HEV in children of Han,Uygur and other ethnic groups were 19.82%(45/227),19.35%(24/124)and 25.71%(9/35)respectively.There was no significant difference between the three groups(χ2=0.735,P=0.692).They were mainly diagnosed with bronchopneumonia,acute upper respiratory tract infection,acute bronchitis and lobar pneumonia and detection rates were separately 18.09%(36/199),24.56%(28/114),18.84%(13/69)and 25%(1/4).There was no statistically significant difference with different diseases(χ2=2.030,P=0.566).The detection rate in all age groups was no difference in group ~3(22.48%),~6 group(22.45%)and ~10 group(22.05%).The detection rates within 1 years(14.63%)and over 10 years old(11.11%)were lower than that in the former three groups,but the difference was not statistically significant(x 2=3.344,P=0.502).Among the HRV/HEV positive children,the ~3 group was highest for 37.19%,and the majority infections of HRV/HEV were within 6 years old,accounting for 80.78%.For season,the HEV infection was relatively high in January(37.21%),May(16%),July(19.40%)and December(15.68%),that is,HEV is mainly prevalent in winter and summer,and HRV was relatively high in March(14.81%),May(16%)and October(14.81%),in other word,HRV is mainly prevalent in spring and autumn.26 in 30 positive samples of HRV and HEV were successfully sequenced,The sequences were submitted to GenBank for Blast and showed that 16 strains were HRV(7 HRV-A,9 HRV-C),10 strains were HEV(4 HEV-CV-A type,6 HEV-CV-B type).According to homology and genetic distance analysis,5 genotypes of HRV-A and 6 genotypes of HRV-C were identified.4 genotypes of HEV-CV-A and 1 HEV-CV-B genotypes were also identified.Conclusions: HRV/HEV is one of the main pathogens of respiratory tract infections in children.There are seasonal differences between the two viruses.HEV is mainly prevalent in winter and summer,and HRV is prevalent in spring and autumn.The results showed that the genotypes of HRV and HEV were complexity and diversity,so it had great significance to strengthen monitoring and prevention. |
PDF Full Text Request