The Effect Of Fucoidan On Proteinuria In Adriamycin Induced Nephropathy Rats

Objective The primary renal diseases was clinically classified acute and chronic glomerulonephritis as well as nephrotic syndrome(NS),of which the NS accounts for 50%.The nephrotic syndrome(NS)is resulted in by diverse factors and its pathologic changes mostly demonstrate the increased permeability of glomerular basement membrane,mass proteinuria and decreased glomerular infiltration rate.The long-term proteinuria may impair kidney function,eventually induce uremia.In this study,the model of nephropathy rats induced by adriamycin(ADR)was successively established.Meanwhile,the effect of fucoidan on model rats was observed and the mechanism of fucoidan was investigated.Methods 35 healthy male SD rats,aged 6-8weeks,weighing(180±20)g,provided by 89 th Hospital of PLA of China,were kept in good condition with temperature of(23±2)℃ and humidity of 40%~50%.All subjects freely take food and drink.After one week of adjustable raising,10 rats were randomly chosen as the control.The remaining 25 rats were given intravenous injection of adriamycin dissolved in saline water(5mg/kg)and their quantity of 24 hours urine protein was tested after treatment in 7 days.The subject with the amount of urine protein>30mg/100 g was chosen as successive model and eliminated the unmeet ones.The chosen model rats were randomly and equally divided into model group(adriamycin)and drug group(Fucoidan + adrimycin).The model group were given intragastric injection of clear water,while the drug group were given with fucoidan(200mg/kg/d)for 5 weeks.The influence of fucoidan on the rat model induced by adriamycin(ADR)on their general state,body mass and the amount of 24 hours proteinuria.Moreover,the kidney tissue histology stained by hematoxylin eosin and Masson was observed by light microscope and the ultramicrostructure by electron microscope,while the expression of fibroblast growth factor 2(FGF2)in kidney tissue was also observed under the light microscope.Results The model group rats became low spirited,slowly and inactive,and the appetite was poor,the hair turned yellow and the urine volume was reduced.Some rats showed diarrhea,ascites and decreased body mass.The pathological histology dyeing hematoxylin eosin and Masson demonstrated impaired glomerular morphology,and gradually mesangial cell hyperplasia,swelling and dropping of podocytes and the increased expression of fibroblast growth factor 2(FGF2)under electron microscope were found.But after 1,3,5 weeks of intragastric injection of fucoidan,the drug group rats showed better condition,more body mass,more urine volume,less proteinuria and less expression of FGF2 than the control group.The two groups had statistical difference(P<0.05).There was no significant pathological changes under light microscope and no extensive fusions of foot processes were observed by electron microscope,which meant better response to the treatment by fucoidan.The expression of FGF2 at various stages showed positive correlation with the changes of proteinuria(r=0.960)accordingly.Conclusion Fucoidan can effectively treat the nephropathy rats induced by adriamycin(ADR)and for model rats,fucoidan can significantly improve the general condition,alleviate the lesion of the kidney tissue,reduce the amount of the proteinuriea,antagonize the expression of FGF2 and take an active part in the protection of kidney by reducing renal injury.Fucoidan is effective in the treatment of adriamycin-induced nephropathy rats and it reduces the expression of FGF2 may be one of its mechanisms.