The Research On Natural Flavonoid Chinese Medicines Anti-influenza A Virus(H1N1)

H1N1is a subtype of the Orthomyxoviridae, influenza virus A. Its antigen iseasy to mutate, so they have very strong virulence and human beings can’t havelasting immunity. Once it outbreak, it will have serious consequences to humans.Drugs currently on the market are mainly for ion channel blockers and neuraminidaseinhibitors, and some virus has shown drug resistance already. Chinese herbalmedicine have broad development prospects in the research on anti-influenza A virusdrugs cause they have many advantages, such as:multiple target points, not prone todrug resistance, little side effect, can be long-term use. And Chinese herbal medicinenot only accumulated rich experience to treat flu, but also has very rich resourses.Flavonoids has the most types in Chinese herbal medicine and plants. This research isstudy the efficacy and mechanism of the natural flavonoids Chinese medicine againstH1N1virus.This study started from computer level, we applicated of molecular dockingtechnology. Through the Discovery Studio we found all8medicine have a certaindegree of binding to HA, NP, NA of H1N1virus. Then we study drug efficacy oncellular level, found that KA-108, KA-110, KA-20have good effect on anti H1N1influenza virus. IC50of that3medicine are3.3μg/ml,9.45μg/ml,13.96μg/ml, inwhich KA-20inhibition rate was the highest, the therapeutic index of KA-108is thehighest, reaching73.54.There’re some experiments on molecular level and biochemistry level to researchthe mechanism of medicine and further verify their efficacy. By use of biacore SPRanalysis test and neuraminidase inhibitors kit, the results showed that, KA-108caninteract with HA; the target of KA-110is HA and NP, but the interaction of NP wasstronger than that of HA; KA-20has inhibit function on NA. Compared withmolecular docking results, it shows some consistency:among the3confirmed antiviralmedicine, KA-108is best combined with HA, consistent with the biacore result;KA-20is best combined with NA, which have same result to neuraminidase inhibitorscreening.Finally, an animal model is built to valid the drug efficacy in animal body.Whether KA-108, KA-110, KA-20can anti H1N1virus, they need further experiment by use the animal model.