Endometrial Layers And Shallow Muscularis Female Progesterone Receptor Expression

Background and purposeEstrogen receptor (ER) and progesterone receptor (PR) is a protein moleculenuclear receptors belong to the nuclear hormone receptor superfamily of steroid, inthe target organ cells is their expression of the major sites may with the correspondinghormone binding specificity thus forming reactions occurring hormone-receptorcomplex that hormones exert their biological effects and thus cause growth of theendometrium. Studies have shown that when ER and PR content of less than ordysfunction can affect the growth of the endometrium.Seriously troubled women ofchildbearing age diseases intrauterine adhesions (intrauterine adhesion, IUA) is due toimproper operation caused by intrauterine its high incidence of endometrial uterinecavity due to injury and did not timely recovery operations triggered by the uteruswall adhesions wounds that loss of normal uterine morphology. Menstrualabnormalities, patients with secondary infertility is the most troubled of the twosymptoms caused by intrauterine adhesions. Hysteroscopic adhesions dissection(transccrvical resectionofadhesions, TCRA) because it is in the visual separation ofintrauterine adhesions, not only more purposeful, but also to reduce the unnecessarytrauma due to the formation of blind separation. Recent studies have also found thatintrauterine adhesions new problem: the separation of moderate to severe intrauterine adhesions after surgery, the patient and the formation of a new intrauterineadhesions, leading to surgical failure. So intrauterine adhesions prevention ofpostoperative adhesions in clinical treatment today is the problem, but also theresearch focus. Many experts use empirical cycle mimic the hormone estrogen andprogesterone to stimulate change in the residual normal endometrial growth and to re-epithelialization of the wound after surgery, in order to achieve the prevention ofpostoperative adhesions intrauterine adhesions isolated relapse. But no experimentsconfirmed intrauterine adhesions dissection able to use estrogen, progesterone andestrogen and progesterone dosage. Functional layer of the endometrium, superficialbasal myometrium (next to the basal layer of the myometrium) researches thedistribution of estrogen and progesterone receptors rarely reported. In this study, thefunctional layer of the endometrium, myometrium superficial basal and estrogen andprogesterone receptor distribution, in an attempt to provide a theoretical basis forsevere intrauterine adhesions dissection of estrogen and progesterone applicationsMaterials and methodsChoice to choose1Samples from2000-2012the inpatient because of cervicaldysplasia uterus patients,30cases of the age of27,45years old, average38.5years.The patients were selected in accordance with the following criteria: cervicaldysplasia, half a year, normal uterus is not taking any other performance-enhancingdrugs (no hysteromyoma, uterine XianJiZheng, endometrial polyps, etc.), the selectedspecimens for the mid and late menstrual endometrial (mean17.5days).2The conventional paraffin embedding, sectioning thick4u Ill, in case ofimmunohistochemical detection.According to the function layer and base layer,shallow muscular histological characteristics of their respective layers.3Detection of ER and PR: three groups of specimens by immunohistochemical SPmethod dyeing, function layer and base layer, shallow at the grass-roots level as thecontrol group.All reagents in strict accordance with the requirements of the steps, ER,PR main position within the nucleus, with organizational structure and clearbackground and the nuclei are palm red for positive, negative for not shaded, thenrandomly selected from each section10high vision observation, distribution packagepositive cell count normal cells, average percentage.According to the percentage of positive cells, respectively,(-) positive cells was less than10%, the positive (+) cells10%-29%,30%～49%(++),(+++) is greater than50%.Results1The distribution of ER and PR in endometrial function layer (30cases/30cases),base layer (30cases/30cases), shallow base (30cases/30cases).The results show thatER and PR in endometrial function layer and base layer and the distribution ofshallow base has no obvious statistical significance (P>0.05).2ER in endometrial functional layer, basal expression levels and in turn reduce thesuperficial muscle, between the two groups was statistically significant (P <0.001)differences.3PR in endometrial functional layer, basal expression levels and in turn reduce thesuperficial muscle, between the two groups was statistically significant (P <0.001)differences.ConclusionER, PR in endometrial functional layer, the underlying muscle and superficialmuscle layer in order to reduce the level of expression, and severe injuries to guideintrauterine adhesions after separation of estrogen and progesterone medication atdifferent levels of the endometrium.