Microbial Community Structure In The Rhizosphere Of Corydalis Hendersonii Hemsl In The Qinghai-tibet Plateau

Qinghai-Tibet Plateau has a unique a zonality plateau climate, which is located in thecentral Asia and China’s southwest, it can also provide the ideal natural material forlaboratory. Corydalis hendersonii Hemsl is considered to be a kind of Tibetan medicine,recently, however, with the deterioration of ecological environment and continuous mining byhuman, it is becoming endangered in Qinghai-Tibet Plateau. Corydalis hendersonii Hemsl isone of the most important vegetation on the Tibetan plateau，so there is an importantecological significance to study the soil microbial community structure and function diversityof rhizosphere for protect and artificial planting it, at the same time we can obtain uniquemicrobial rhizosphere resources of Corydalis hendersonii Hemsl firstly, and providetheoretical data to identify and establish microbial repository. In this study, we chose11soilsamples along the different altitude, longitude, latitude in the Qinghai-Tibet Plateau to revealthe microbial community structure by using Real time QPCR、Biolog and high-throughputsequence. Combined with the data of soil physicochemical property,the major conclusion andresults were abstracted as follows:1. In the whole of study region, the range of soil rhizosphere pH value is between6.7and7.7, in other word, pH value is generally greater in the middle than both sides, the lowestpH value in the longitude as82.18.828is6.74. The highest pH in longitude as86.35.064is7.68. From west to east, soil C/N value formation decreases gradually, the conductivity of thesoil is significantly reduced. To the best of our knowledge, the soil physicochemical propertyindicated that soil tending to impoverishment and salinization from east to west.2. The quantity relations of the microorganism gene copies were: bacteria>archaea>fungi,with longitude span, the11sample from west to east across15longitude, the gene copiesrange of bacteria was between9.64E+09～8.18E+08, there was a decrease tendency. However,the gene copies range of archaea was as follows:1.22E+05~2.89E+07, form the increasingtrend in turn. The gene copies range of fungi was between2.44E+06～7.22E+04, thetendency was similar to bacteria. On the whole, there was a decrease tendency of microbialgene copies from west to east. By analyzed the results, we found that three parts of microbialshow a different tendency, which implied that soil microorganisms community structure wascomplex. There was a kind of competition or interactions within the phenomenon.3. Using Biolog EcoPlateTMapproach to study microbial community level function, theresults demonstrated that the ability of utilized carbon resource of11soil samples along thelongitude is different. In this study, there was a significantly positive correlation between theability of utilize six carbon resource and electricity, TDS, soil C/N ration. The pH had a significantly negative effect on diversity-H, which implied that soil microorganisms diversity-H was more easily to be disturbed by pH. At the same time, there was a significantly positivecorrelation between diversity-U and TDS, soil C/N ration, which indicated when the carboncontent was higher than nitrogen content, these physicochemical property promote therhizosphere microorganism of metabolic activity, was also more conducive to grow forCorydalis hendersonii Hemsl on the ground. The results show that the diversity ofrhizosphere microorganisms more vulnerable to the effects of soil physical and chemicalproperties than the gene copies of Corydalis hendersonii Hemsl rather than longitude.4. High throughput sequence: there was a significantly tendency that Shannon indexgradually increased from west to east as the longitude. However the Read Number, OUT,chao1and Simpson index reached a peak in sample site of five. Another evident tendency wasthat the number of acidobacteria gradually increased, in contrast the number of bacteroidetesgradually decreased; besides, the number of acidobacteria in1sample site was significantlylower than in2sample site; However, this tendency had been changed firstly, which thenumber of acidobacteria was higher than bacteroidetes in3sample place. Even in4samplesite, the number of acidobacteria was far beyond the bacteroidetes, this advantage had beenpresent in5and11sample sites. So in the large geographical spatial span, there was differenttrend of microbial community in different sample site. It indicated that geographical patternand different environmental factorsaffecting microbial-mass and species composition. Thereis maybe exist intraspecific competition and mutual relationship.