Preparation Of Gold And TiO 2 Nanocyclic Channels And Their Application In The Separation Of Penicillamine And Other Drugs

Nanochannel is the pores or tubles with diameter from 0.1 to 100 nanometers. As an important branch of bio-nanotechnology research, nanochannels play an important role in the separation of biological materials, organic small molecules and chiral substances. The separation and detection of objectives will be greatly promoted because the characteristics of nanochannels can be significantly changed by chemical modification.In this paper, the gold and titanium dioxide nanochannels were prepared and characterized. The transportation of drugs through the functionalized nanochannel membranes was investigated. Compared with the traditional high performance liquid chromatography and capillary electrophoresis, there are few reports on the application of nanochannel technology to separate drugs. Details are presented in this thesis as follows:(1) Au was deposited within the pores of the polycarbonate template membrane using an electroless deposition method. Au nanochannels were characterized by scanning electron microscope(SEM), infra-red spectrum(IR) and so on. L-cysteine was modified onto the gold nanochannels based on the Au-S bond. Because of the chiral sites of L-cysteine, it can recognize penicillamine enantiomers. We studied the seperation of penicillamine enantiomers through gold nanochannel under different conditions, and compared the advantages and disadvantages of several chiral separation methods.(2) Titanium dioxide was deposited within the pores of the polycarbonate template membrane using a liquid phase deposition method. Titanium dioxide nanochannels were characterized by SEM, transmission electron microscope(TEM), IR and so on. Under different p H, the electrical property of titanium dioxide nanochannels were studied. Several methods of the preparation of titanium dioxide nanochannels were compared.(3) The titanium dioxide nanochannels were used to study the transportation of folic acid through the membrane. At p H = 7.4, the transportation rate of acetyl salicylic acid, folic acid and BSA was studied. The effect of acetyl salicylic acid and BSA on the transportion rate of folic acid was investigated. The effects of the concentration of BSA, p H and ions on the transportion rate of folic acid through the membrane were discussed.The separation and analysis technology by nanochannel methods proposed here provided a new idea for biological molecules separation and analysis.