The Research Of Resveratrol Anti-AD-like Nerve Inflammation

Objective:The current study was designed to investigate the effect of resveratrol(Res) onAD-like neuroinflammatory changes induced by lipopolysaccharide(LPS) in rats, andfuther explore possible mechanisms of action.Methods:Eighty-five male Sprague-Dawley (SD) rats were randomly divided to sixgroups:control, LPS, LPS+Ibuprofen (IBU,40mg/kg as a positivecontrol), andLPS+Resveratrol(Res10,20,40mg/kg) groups. To establish inflammatory model,rats inthe LPS-stimulated groups were administered with LPS by intraperitoneal injection (i.p.) atthe dose of0.5mg/kg once weekly continuously for13weeks. The weight and mortalityin rats was recorded daily.Morris water maze was used to detect the cognitive function.HEstaining and electron microscope was performed to observe neuronal morphology inhippocampus. The expression of inflammatory factors (COX-2,TNF-α,IL-1β and TGF-β1)and its regulatory proteins (p-p38,p-ERK1/2,p-JNK,IκB-α,p-NF-κB-p65and p-Akt)wasanalyzed by western blot.Conclusion:Res could significantly attenuate AD-like neuroinflammation changes inducedby LPS in rats,and the mechanism may be related with the inhibition of proinflammatorycytokines production and p-p38,p-ERK1/2,p-JNK and p-NF-κB-p65activity, as well as theactivation of IκB-α and p-Akt activity.