Synthesis And Its Activity Of The Fluorescence Probe Based On Transition Metal Complex

Fluorescence analysis method is an analytic method based on fluorescence probe,which has the advantages of high sensitivity, high selectivity, various measurementparameters and techniques and so on. In recent yreas, more and more researchers inbiology and medicine show their interests in the method. As a normal probe used influorescence analysis method, transition metal complex fluorescence probe is superiorto other probes in some properties, such as long fluorescence lifetime, high sensitivity,large Stokes shift, excellent light stability and so on. Nowadays, the probe has been agreat development in biochemistry, medicine analysis and environmental monitoring.In this paper the research work mainly includes the following aspects:1) Based on the synthesis of nickeal (II) complexNi(EDTB)]·2ClO4·2CH3CH2CH2CH2OH [EDTB=N, N, N’, N’-tetrakis-[(2-benzimidazolyl)methyl]-1,2-ethanediamine], effects of the complex in the hydrolysis ofurea have been studied with fluorescence analysis method. The results indicated that thecomplex had catalytic activity in the hydrolysis of urea. Furthermore, we had discussedthe catalytic mechanism of the complex in the hydrolysis of urea with massspectrometry, which well simulating the function of urease.2) The copper (II) complexes [Cu(IDB)2]Cl2·2CH3CH2OH·2H2O [IDB=N, N-di(2-benzimidazolylmethyl)imine](1) and [Cu(EDTB)]·2[C6H4(OH)COO]·6H2O (2) havebeen synthesized and the crystal structures have been determined by single crystalX–ray diffraction. The crystal structure analysis of complex (1) shows that thesix–coordinated copper (II) is a distorted octahedron pyramid with four benzimodazolenitrogen atoms and two amine nitrogen atoms of two ligands IDB. The crystal structureanalysis of complex (2) shows that the six–coordinated copper (II) is a distortedoctahedron pyramid with four benzimodazole nitrogen atoms and two amine nitrogenatoms of the ligand EDTB. Both of the complex (1) and complex (2) are of fluorescentproperties, the maximum emission wavelength of which are445nm and572nm,respectively.3) In non-buffer system (aqueous solution) and different buffer systems (0.01mol L-1phosphate buffered solution of pH=7.4and0.1mol L-1ammonium carbonatebuffered solution of pH=8.9), biotin-rhenium(I) complex [Re(N-N)(CO)3(py-CH2-NH-biotin)](PF6)[N-N=2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline; py-CH2-NH-biotin=N-[(4-pyridyl)methyl]biotinamide](Re-Biotin) used as the QTL probe (quencher-tether-ligand, QTL), the fluorescence quenching of water-soluble anion fluorescentpolymer poly[5-methoxy-2-(3-sulfopropoxy)-1,4-phenylenevinylene](MPS-PPV) after adding Re-Biotin and its fluorescence recovery after adding streptavidin wasinvestigated. The mechanism of fluorescence quenching and fluorescence recovery ofMPS-PPV was discussed.The results indicated that in non-buffer system and different buffer systems, thefluorescence quenching effect could be interpreted as the strong electrostatic interactionand charge transfer between Re-Biotin and MPS-PPV. Its fluorescence recovery afteradding streptavidin could be interpreted as the specific interaction between biotin ofRe-Biotin and streptavidin pulling Re-Biotin away from MPS-PPV. Based on this, astreptavidin sensor was developed by using water-soluble fluorescent conjugatedpolymer MPS-PPV as sensitive material. And the sensing mechanism is stable innon-buffer system and different buffer systems. Streptavidin was detected with thisnovel class specific, rapid and highly sensitivive method.