Design And Implementation Of The Optical Structure Of A Miniaturized Surface Plasmon Resonance Biosensor

Surface plasmon resonance (SPR) is a kind of detection technology based onlight-physical phenomenon. The samples need not be marked, isolated and purified. Itcan not only detect the interaction between the biological macromolecules on line and inreal time, but also ensure the type and concertration of reactants. SPR technology hassome advantages such as high speed and high sensitivity, so that it is used extensively inbiology, chemistry, medical treatment, pharmacy environment and food analysis.At present, the commercial SPR biosensors are high cost and non-portable, so it isdifficult to be used widely. With the continuous development of SPR detectiontecnnology, miniaturized and portable SPR biosensors become the new trend. The mainaim of this subject is to design the optical structure of a miniaturized SPR biosensor, setup a SPR sensor experimental platform, and detect the single sample.At the beginning of this paper, the application, research situation anddevelopmental trend of the SPR detection technology are introduced, and then therelated basic concept and principle are discussed in detail. The producing condition ofSPR is analysized through some theoretical formula. The temperature characteristics ofthe SPR biosensor are also researched in theory, including the impact on the light source,prism, metal film, solution and optical detector. A five-layer-film optical system isdiscussed by a numerical analysis, and the influence of SPR curve caused by thewavelength, dielectric constant and thickness of the metal film, and the refractive indexof the prism and the analytes are simulated. And then, the whole SPR sensor system isintroduced. In order to realize miniaturization, a new prism is designed, and the lightsource and the detector are on the same plane by utilizing the reflector, thus the volumeof the device is reduced. The experimental data collection, storage and display can berealized by the data acquisition module and the corresponding software.Air, water and different concentration of ethanol solution are detected by theself-made SPR sensor, and these SPR curves are obtained correspondly. Through theanalysis of the experimental data, it is clear that, with the increase of the concentrationof the analyte, its resonance angle is also bigger. This device can be utilized to detect thesingle sample with preferable linearity and stability.