Clonostachys Rosea Induced Non-host Resistance To Gray Mould Disease In Tomato

The tomato gray mould, which is one of the major diseases in tomatoes protected cultivationaround the world, is caused by Botryis cinerea. This disease can infect tomato leaves, stems,flowers, fruit and other parts, which mainly is fruit damage, spreading rapidly after the onset of it,and it has done great threaten to the tomatoes protected production. Tomato gray mould at presentin all over the China, have become the barriers of sustainable development of tomato in protectedcultivation.Domestic and foreign scholars have attached great importance to the occurrence of graymould and prevention and control work, a lot of innovative research work had been done in manyaspects, but because there is no source of resistance to B. cinerea, resistance breeding is verydifficult to continue. At present, the measure of tomato gray mould controlling in the productionprocess is mainly chemical control. But because of the irrational use of chemical agents, pathogenresistance occurs rapidly with it and the control effect decreased year by year. In addition, as themain target application is the fruit, using the chemical agents can cause serious pollution ofpesticides in fruit and environment also can do harm to the health of human and animal. Therefore,people turn sight to the biological control methods in the control of B. cinerea. With the researchon biological control of B. cinerea is increasingly deepening, a lot beneficial microorganisms thathave B. cinerea resistance and their metabolic products have been screened and utilized.Clonostachys rosea is a common biocontrol fungi, plays an important role in biocontrol ofplant diseases. Research shows that, antagonistic mechanism of C. rosea is diverse, in addition tothe hyperparasitism, competition, antibiosis and cell lysis, also have induced plant systemicresistance. Although the related research of C. rosea induced resistance mechanisms have beenreported, it still not perfect. A thorough study of Gliocladium roseum induced resistant mechanismis conducive to the prevention and control of plant diseases.The main results were obtained as ofllowing:1. This study isolated and purified the strain t08016b from infected tomato fruits with graymould, and comprehensive morphological identification, analysis of the18SrDNA,5.8SrDNA-ITS,rDNA sequence and pathogenicity analysis, identified as B. cinerea.2. Analyzed and assayed signaling molecules, endogenous hormones on tomato plant anddefense related enzymes after different treatments on tomato fruits with spore suspension of the laboratory preservation of C. rosea and strain t08016b.The results showed that there were significant differences in resistance response to tomatogray mould with different inoculation treatments. Comprehensive analysis of the variousparameters, definitely the C. rosea induced tomato to produce a non-host resistance, and there maybe a superposition effect between non-host resistance and host resistance. Among them, it caninduce plant resistance better on tomato fruits when inoculated with B. cinerea first and then C.rosea inoculation, changes of the signal molecular, plant hormones, defense related enzymes arethe biggest meet the need of plant disease resistance; tomato fruits inoculated C. rosea only canchange related factors smoothly at low amplitude, it can apply the C. rosea as a "plant vaccine" inagainst gray mould disease; tomato fruits inoculated B. cinerea only, also can induced plantresistance response, but this weak resistance cannot ward off invasion process of B. cinerea.3. Comprehensive comparative analysis of proteome under different treatments showed that,superoxide dismutase and polyphenol oxidase upregulated express in various inoculated methods,and this illustrates both these two enzymes are involved in plant disease resistance process; tomatofruits inoculated B. cinerea only and inoculated C. rosea only had its different up-regulated anddown-regulated proteins, it was showed that the tomato has different path in response induced bytwo kinds of microorganisms, namely, C. rosea induced plants produced the non-host resistance;ATP synthase alpha subunit protein is kind of protein that would upregulated express onlyinoculated with C. rosea, which is special compared with the only inoculated B. cinerea, may be akey protein of nonhost resistance in plants.