The Effect Of Taurine On The Nonspecific Immune, Digestive Enzyme Activity And Antioxidant Ability In The Tiger Frogs(Hoplobatrachus Rugulosa)

The decline and extinction of global amphibians, which is thought to be a bellwether for environmental degradation, have led to public concern in recent years. And there are lots of factors causing these declines, such as habitat loss, over-harvesting, pollution, increased ultra-violet irradiation as well as disease, are probably at work. The amphibian with the special morphological and biological characteristics was seemed as the indicating species which can reflect the changes of the environment in some extent. The taurine with the functions of regulating the cellular pressure and sustaining the common lipid metabolism and enhancing the antioxidant and immune ability which spreads widely in organism is an essential amino acid. The immune system which is very useful for the living of the organism has played an important role in the aspect of resisting the pathogen and protecting the organism, and can sustain the stability of the body. The occurrence and development of disease is concerned with low immune ability and immunodeficiency. Therefore, it has attracted importance to regulation of immune ability and the treatment of disease. While, the research information which is about the effect and mechanism of the immunology and stress factors is less in amphibians, and there is no public material about the effect of taurine on the immune ability of tiger frogs in domestic and overseas. The taurine, a body’s metabolism product containing sulfur, which is related with the immune and widely biological functions fin animals, is an essential physiology activator.The tiger frog whose wild population has declined sharply is an economic and edible animal in the south of China. In order to meet the demanding of tiger frogs in our daily life, the breeding work has developed in the world. The fact is that mass mortality phenomenon has appeared during the process of breeding, which is cased by diseases. Therefore, the tiger frog breeding work is still in the primary and exploring stage. The aim of our research is to study the effect of taurine on the nonspecific immune and antioxidant ability and enzyme activity through establishing the measuring methods of nonspecific immune index and gastric lavage in the tiger frogs. The chief results were listed as follows.1. The methods of detecting nonspecific immune index have been established and these indexes have been adopted to evaluate the changes of immune indexes and the intestine and liver enzyme activities with different does of taurine solution (0mg/ml,0.2mg/ml,0.4mg/ml,0.8mg/ml,1.0mg/ml,1.4mg/ml,1.8mg/ml,1ml/50g). The results indicated that the gastric lysozyme activity appeared to be an increasing trend with1mg/ml taurine solution (p<0.05). NBT response intensity and fluorescence intensity have been enhanced with0.8mg/ml. The MDA content in blood appeared to be a decreasing trend, and dropped to the lowest level with the concentration of0.8mg/ml (p<0.05). The GSH content expressed to be an increasing trend firstly and decreased subsequently, and the peak appeared with the concentration of1mg/ml (p<0.05).2. In order to study the digestive process of taurine in tiger frogs, the glucose content in blood and liver, and the enzyme activities in liver and intensity have been detected after pouring taurine solution (0.8mg/ml,1ml/50g) during the different period (0h,1h,2h,3h,4h,5h,6h,7h,8h,10h,12h). The results reflected that the activities of protease and amylase and lipase have presented to be an increasing trend firstly and a decreasing trend subsequently. The enzyme activity is distinctive higher with the taurine solution (0.4-1mg/ml) compared with other concentration, while high taurine concentration would inhibit the enzyme activity; taurine would be absorbed during the period of ten hours after gastric lavage in the tiger frogs; the protease activity of intestine and liver reached the peak after three hours and seven hours respectively(P<0.05); the liver lipase activity reached to the peak after three hours(P<0.05), and the peak appeared in the third hour(P<0.05). The effect of taurine on the glycogen in liver and blood was remarkable, hepatic glycogen content increased to the peak rapidly after one hour and then dropped, and the glycogen content in blood dropped to the lowest level after three hour(P<0.05).We can concluded that the effect of taurine on physiological indices is meaningful for the breeding work of tiger frogs, and this study enriched the immune material of frogs especially for the valuable frogs. Furthermore, the study can also provide technical guidance for the artificial breeding of tiger frogs in some extent.