The Research Of Pharmacodynamics And The Establishment Of Bioassay Tests About Ossotide Injection

Purpose:Study of Ossotide Injection treat primary osteoporosis disease and promote proliferation role to Osteoblast. Build Bioassay Tests about Ossotide Injection.Method:60health only female SD rats resection bilateral ovarian30days after the randomly divided into blank control, model group, and the Nilestriol Tablets group, Ossotide Injection of high, medium and low group, each only10, and blank control group and model group injected with equal physiological saline, each group is continuous to medicine12weeks; In vitro culture Wistar rat cranial cover breast bone osteoblasts, MTT method to detect proliferation of Ossotide Injection.Wistar rat cranial cover the bone osteoblasts training and the generation of human osteoblestlike cells MG-63training method.Through the MTT method to detect and build Bioassay Tests about Ossotide Injection.Result:1Ossotide Injection can improve no ovarian osteoporosis rat models of lumbar spine, of the decline of the femur bone mineral density, reduce blood decompensated elevated alkaline phosphatase, on the osteoporotic model rats wet weight, bone dry weight, to fat dry weight, weight, bone ash calcium, phosphorus has obviously improved,to reduce serum levels of estradiol content and uterus index of a certain improve but reduce not statistically significant;HE dyed light-microscopy visible bone trabeculae structure broadens, and bone trabeculae number increase, the structure arrangement of normal form and near, the connections between the trabecular meshwork, bone marrow cavity narrowed, bone marrow tissue and bone trabeculae area than smaller, bone trabeculae osteoblast gradually increase the number of surface, the arrangement more closely, the marrow cavity broken bone cells and its precursor gradually reduce; Electron microscopic bone trabeculae complete structure, at high magnification is covered on the surface of the bone trabeculae collagen cellosilk even thickness, cellosilk to arrangement between the rules, neat and close to, the collagen fiber silk is done, in the absorption near lacuna, collagen cellosilk a tidy, fracture less, collagen arrangement tend to normal. Success in the separation, purification and cultivate Wistar rat skull, and identified as osteoblasts, after MTT test show that Ossotide Injection can obviously promote proliferation role. 2Using Wistar rat skull,resemble human osteoblestlike cell lines MG-63to examinetheOssotide Injection to effect bone cell proliferation,sure5x104cellular density of0.1mg/mlof administration dosage,training72hours as the bestconditions,the results show thatOssotide Injection has the obvious effect for cell proliferation and build Bioassay Tests aboutOssotide Injection.Conclusion:Show that Ossotide Injection has fight osteoporosis and remarkablecurative effect,Promote bone cell proliferation obviously.Build Bioassay Tests about OssotideInjection.