The Protective Effect Of Tempol On Skin Photoaging Caused By UVB In Harirless Mice

Objective:To investigate the protective effect of Tempol on skin photoaging caused by UVB in hairless mice.Methods:Seventy-two hairless mice were divided into normal control group (Group A), UVB radiation control group (Group B). topical use of Tempol and radiated after an hour group (Group C), topical use of Tempol and radiated after two hours group (Group D), topical use of Tempol and radiated after four hours group (Group E) and topical use of Tempol and radiated after eight hours (Group F). 0.11J/cm2 UVB was given every other day. The total time was 14 weeks and the total dose was 5.39J/cm2 The content and histological changes of collagen and elastic fibers in the dermis were observed with the microscope. The MDA and HYP content changes in hairless mice skin were assayed with the method of biochemical analysis.Results:The collagen and elastic fibers in the dermis of hairless mice in group B, C, E and F became thicker, fragmented and disarranged. The malondialdehyde (MDA) content was significant increased in group B, C, E and F. The differences between group B, C, E, F and group A, D was significant (P<0.01). The hydroxyproline (HYP) content was significant decreased in group B, C, E and F. The differences between group B, C, E, F and group A, D was significant (P<0.01).Conclusion:topical use of Tempol can alleviate the skin photoaging of hairless mice caused by radiation with UVB, and its protection was affected by time. The best effective time is about 2 hours after topical use.