Based On Several New Types Of Nano-au Glucose Biosensor

Several novelty amperometric glucose biosensor based on gold nanoparticles are described in this work. The dissertation consists of two parts.Part I reviews the development and application of the amperometric glucose biosensor and nanoparticles in recent years.Part II is experiments which have been divided into four chapters. Chapter 1 studies a glucose biosensors fabricated by self-assembling of double layer 2d-network of (3-mercaptopropyl)-trimethoxysilane (MPS), gold nanoparticles and glucose oxidase (GOD) on gold substrate. A clean gold electrode was first immersed in solution of MPS in ethanel to produce a self-assembled monolayer, then the silane unite were polymerized into a 2d-network by dipping into aqueous of NaOH. The second silane layer was then formed by immersion back into the MPS solution overnight, and then the gold nanoparticles were chemisorbed onto the thiol groups of the second silane layer. Finally, GOD was adsorbed onto the surface of the gold nanoparticles. The modified process was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Analytical parameters such as pH, temperature were also studied. With the aid of Co(bpy)₃³⁺ as a mediator in the solution, the electrode displayed excellent electrocatalytical response to the glucose. The peak currents were proportional to the glucose concentration in the range of 4.00×10^-10 to 5.28×10⁽-8mol/L with a correlation coefficient of 0.99. Moreover, such a modified electrode exhibits a good stability and sensitivity.Chapter 2 reports a glucose sensor prepared by immobilizing of glucose oxidase and nanoparticles in polyvinyl butyral (PVB) on a Prussian blue-modified platinium electrode was described. The properties of the sensor were investigated.Chapter 3 reports a novel glucose biosensor immobilized by self-assembling polymer of Prussian blue and 1,2-diaminobenzenes (1,2-DAB), gold nanoparticles and glucose oxidase (GOD) on gold substrate. A clean gold electrode was first immersed in solution to produce a self-assembled layer of PB, then electrosynthesis of...