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Using A Series Of Material Nano-gold Particles As The Electron Mediator To Enhance The Study Of New Glucose Biosensor

Posted on:2005-10-05Degree:MasterType:Thesis
Country:ChinaCandidate:Q F LiFull Text:PDF
GTID:2208360122992779Subject:Analytical Chemistry
Abstract/Summary:PDF Full Text Request
Diabetes mellitus is a common and frequently-occurring disease, which seriously menaces the healthy of the human beings. Therefore, the diagnosis and treatment of diabetes mellitus is a heavy responsibility for the biomedical engineering field of the world. Glucose biosensor is the major technique for glucose analyses in clinical practice. For the advantages of glucose biosensors are obvious: high specificity, short time, simplicity and low cost of analysis, etc. One of the developments of glucose biosensor is the third generation amperometric enzyme electrode with direct electron transfer, which is commonly prepared by conducting organic salts and conducting polymers. Development of a third generation enzyme electrode is one of the long-term goals of biosensor program in the world. It is very necessary to search new methods to prepare the third generation biosensor. Another development of biosensor is miniaturization and integration. For the purpose, glucose biosensors have to overcome the problem topic: to enhance the current response. Therefore, it is very essential to explore new materials and methods for preparation of new direct electron transfer biosensor with high response.In the present thesis, we make use of hydrophilic gold to immobilize enzyme, investigate the use of colloidal gold for amperometric biosensor. At the same time, we take advantage of some substances those have electroactivities as electron transfer mediators with gold nanoparticles to compose novelty glucose biosensors. Nanoparticles can play an important role in adsorption of enzyme due to the large specific surface area and high surface free energy, at the same time, there is evidence that enzyme adsorb tightly to the surface of the gold particles while retaining their enzymatic and electrochemical activity. Although the electrodes exhibited excellent stability and long lifetime, the operation potential of glucose determination may introduce interference in real biological samples. In order to overcome this drawback, the electron transfer mediators were explored, we select Nathpol Green B, Azure, tris-2, 2'-dipyridylcobalt(III) perchlorate trihydraie in glucose solution as electrontransfer mediators to improve the glucose biosensor's sensitivities and linear scope. The result of our experiment indicated the novel biosensors exhibite excellent electrochemical characteristics to glucose with substantial improvement in sensitivity, low detection limit, stability and anti-interferer. And the result of determination of the human serum is satisfied.
Keywords/Search Tags:glucose biosensor, cyclic voltammetric, gold nanoparticles, electron mediators.
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