Separation Of Atnhx1 Gene Validation, And Salt Tolerance

Salt injury was one of the main abiotic stress factors which impacted the plant growth and development. There were plenty of saline soil and secondary saline soil on land surface. How to make use of large areas of saline land for agriculture production was a problem to be solved urgently. Cultivating salt-tolerant crop varieties was a hot spot of life science research. Glycophyte lacked main effect salt- tolerance gene and we hoped to cultivate salt-tolerant species by genetic engineering means. In this study, the tonoplast Na~+/H~+ antiporter gene AtNHX1 with a salt-tolerant major gene characteristic was cloned from the Arabidopsis by RT-PCR method. The dicotyledon expression vector which contained AtNHX1 gene was constructed and AtNHX1 gene was transformed into model plant tobacco by Agrobacterium-mediated Transformation in order to verify the salt-tolerant function of AtNHX1 gene. The results were as follows:1. The PCR primer was designed by the sequence of Arabidopsis AtNHX1 gene which published by Gaxiola (1999). The AtNHX1 gene was isolated by PCR cloning method with the cDNA of Arabidopsis as template and the plant expression vector pBI121*-AtNHX1 was constructed.2. Agrobacterium-mediated foreign gene genetic transformation system for tobacco SR-1 was optimized. The AtNHX1 gene was transformed into tobacco by optimized transformation system and 57 transgenic tobaccos from independent resistance buds were obtained. Molecular detection showed that the AtNHX1 gene was integrated into tobacco nuclear genome and expressed.3. The salt tolerance of transgenic tobacco of AtNHX1 gene was significantly enhanced. We should try to transform rice after modified the gene in order to create salt-tolerant transgenic rice materials.