Fission Yeast Sod2 Gene Cloning And The Cultivation Of Transgenic Arabidopsis

Mn plants are exPosed to salhaty the ions, tyPicaily Na+ reduce theaPoplastic Waer poteniial and accumu1ates excessively in the cytoplasm. Plamcell adaPtS to the sait stress through accumulation of osmotic solthes and ionhomeostasis modulation by compartmentalizing Na+ in vacuo1e or activating theeffiux systeIn of Na+. AtNAN encoding a vacuolar Na+/H+ aniporter and SOS1encoding a plasma membrane Na+/H+ antiporter were isolated from glycophyteArabmpis thNa, and overeXPression of Arm in ArabmpiS thalianaincreases the salt tolerane of transgenic plans sighficanly. Gene engineeringbased on reducing Na+ toxicity creats a new aPproach to plan salt-tolerance.SOD2 from Schizosaccharomyces pombe responses tO effiux of Na+ and it is anmpoM H relating to homeostalis. Thus. overexPresion of SOD inArabmpis thaliana maybe edute tO the ion homeoStaSis in the cell.The SOD2 gene was iso1ated from Schedmyces pombe byRT-PCR Strategy and condrmed by sequencing. But difference was foUnd atfour bases of the sequence from the previous reports. The cDNA of meencodes a protein of about 51KD and composed of 1404bp' Then, aintegrated vector of SOD2 and NPt II of pROK2 plasmid was constrUted andintrOduced into Arabmpis thaliana by in plhata transformation methodmediated by AgThbacterfum tumeAscjens Transformans were screened bytheir abi1ity of growing on media containing kanamcin. The genetransfOnnation was coford by PCR analysis.