Study On Fermentation Technology And Separating Of Lipase With Candida Sp.99-125

Lipase from Candida sp. is one of important microorganism enzymes in industrial application. In the present work, the fermentation production of lipase with Candida sp.99-125was studied. By optimization of medium, soybean meal was chosen as nitrogen source. The highest lipase yield was5000IU/ml at26℃,220rpm and no adjusting of initial pH.Effect of soybean meal hydrolysate as a nitrogen source on lipase production was investigated. And we found that soybean meal hydrolysate was favorable for lipase separation and extraction which made it more competitive than soybean meal. Furthermore, the effect of lipid on lipase production during fermentation was investigated, too. Several soybean oil metabolites were analyzed and change of them was recorded too. The results indicated that as a metabolite of soybean oil, oleic acid could induce the production of lipase, which was important for improvement of lipase activity.The process was scaled up from the laboratory bioreactor (30L) to two pilot fermentors (100L&1m3). The lipase activity reached8000IU/ml when broth pH was controlled by feeding of soybean oil. In the lipase separation process, best ratio of coarse lipase powder to water1:7was obtained which made the yield as high as97.8%. The best surface-active agent was0.15%Tween60which made the yield as high as83%. In the process of wet lipase treatment, higher lipase activity was obtained when dried at room temperature rather than vacant evaporation.