Cloning And Function Analysis Of CiPP2C37-like Gene From Caragana Intermedia

Plants often encounter stress factors such as drought、high salt and low temperature in the process of plant growth and development. Plants growth was inhibited and even lead to plant death in severe cases. Plants must adapt to abiotic stress by adjusting its metabolism. Phosphorylation and dephosphorylation of protein is an important reaction type in the process of cell life. It is the main way of protein post-translational modification. Biological regulate its life activity by this system. PP2C is an important protein phosphatase, which main function is respond to abiotic stress by participating in different signaling pathways. Caragana intermedia plays a crucial role in drought resistance, cold resistance, improve soil nutrient, protect and restore the ecological balance. In this study, CiPP2C37-like gene middle fragment was obtained from pre-constructed Caragana intermedia SSH library, CiPP2C37-like encoding gene was cloned, bioinformatics, gene expression and related functions are analyzed. The main results are as follows:1. CiPP2C37-like gene 5’and 3’sequence were cloned by rapid amplification of cDNA. The Amplification length respectively is 1158bp and 505bp. The full-length cDNA of CiPP2C37-like was 1734bp with the 5’UTR of 170bp and the 3’UTR of 334bp. The ORF of CiPP2C37-like was 1230bp, encoding a protein of 409 amino acids which was hydrophilic.2. Real-time-quantitative PCR analysis showed that 100uM ABA,300mM NaCl, dehydration and 4℃ low temperature stress enhanced CiPP2C37-like gene expression at the transcriptional level, indicating that CiPP2C37-like may be involved in these stress resistance molecular mechanisms of Caragana intermedia.3. CiPP2C37-like gene overexpressing Arabidopsis seeds germination rate were significantly higher than the wild type under 10%,15% PEG6000 and 200mM NaCl stress treatment.4. CiPP2C37-like gene overexpressing Arabidopsis lines enhanced tolerance to salt and drought, as compared with wild type under 200mM NaCl and drought stress treatment.5. The seeding of CiPP2C37-like gene overexpressing Arabidopsis lines were smaller than wild type in 1-3 weeks and the seeding size of overexpressing Arabidopsis lines and wild type were consistent after four weeks under normal growth condition.