Research On SPR Sensor And Its Application In Studying The Interaction Between β-cyclodextrin And Azobenzene

Surface Plasmon Resonance (SPR) technology, a new sensing technique, was firsly reported in 1980s, involving the knowledges of optics, chemistry, electronics, etc. The technique possesses many merits, such as real-time and swiftly-response, high-sensitivity, unpollution, label-free, need-less-sample, just because of which, it has been becoming a leading technique for biomolecular interaction analyses during the past two decades. In particular, the technique has been developing tremendously since its application in the life sciences in the middle of 80s. Nowdays, the application of the sensors based on SPR technology have been found in a variety of fields such as optics, medicine, pharmaceutics, environmentology, and so on in many countries (USA, Japan, Netherlands, Sweden,etc).The main works in this thesis are (1) to design and construct an integrated differential SPR sensor system. and select a system to validate the feasibility of this differential SPR sensor. Based on the principles of SPR, we compiled a program in LabVIEW language to simulate numerically the conventional SPR response curves and obtained optimal parameters for SPR sensor's design and debug. We have prepared both the hardware and software of SPR sensor, constructing the sensing system. Testing the dependence of the resonance angle shift on the concentration of ethanol shows that the SPR sensor works very well. (2) to employ the SPR sensor constructed by our own to study the interaction betweenβ-cyclodextrin and azobenzene. Firstly, we synthesized thiolatedβ-cyclodextrin for the purpose of self-assembly monolayer on surface of gold film. Next, the inclusion interaction betweenβ-cyclodextrin and azobenzene was studied with spectroscopy and electrochemistry(Square Wave Voltammetry, SWV), offering the results which would be used for comparison with SPR result. Finally, observation on the interaction betweenβ-cyclodextrin and azobenzene was carried out by using SPR sensor, indicating that the results by SPR are in good agreement with that by spectroscopy and electrochemistry. It is worth noting that the detection limit of azobenzene with SPR is as high as 10-13 M.The innovation points in this dissertation are two: (1) The differential meaurement of SPR signals are realized with a quadrant photodetector and differential amplification circuit, eliminating the errors systematic drifts and solution bulky effect (2) With a gold film modified withβ-cyclodextrin, the SPR sensor is conveniently used for real-time detection of azobenzene in trace levels...