Study On The Preparation And Performance Of The New Fluorescent NO Fiber Optic Biosensor

Nitric oxide (NO) is an important biological messenger molecule with free radical property; it has important physiological functions in the nervous, immune, blood, endocrine, respiratory, digestive and other systems; and also plays an important factor in the pathological process of cardiovascular diseases, diabetes, cancer, obesity and other diseases. Therefore, it is of great significance for the detection of NO in vivo for the life sciences, basic medicine, clinical medicine and other fields. Fiber optic sensor is a trace analysis technique with high sensitivity and fast signal transmission for the past ten years. Because of the unique advantages of large information transmission capacity, excellent environmental adaptability of electromagnetic interference and shock, multi-component sensor integration, easy to be miniaturization and can be monitored online with remote sensing in situ, etc., the fiber optic sensor represents the development trend of a new generation of sensors, and has great potential for application in health care, water quality monitoring, food inspection, biological agents testing, etc. The NO sensor makes by optical fiber sensor technology, with high sensitivity, fast signal transmission, excellent environmental adaptability, and easy to be miniaturization, which attracts many scientists'attention.This paper was supported by the National Natural Science Foundation of China (NO.50802069) and the Fundamental Research Funds for the Central Universities (2010-IV-050). We synthesized two kinds of NO sensitive materials:vicinal diaminobenzoacridine (VDABA) and 9-(4-(1,2-diamine)benzene-N1-phenyl)acridine (DABPA) from the material design, structure, properties and biological detection, and then embedded NO sensitive materials on the optical fiber probe, designed and built a new fluorescent NO fiber optic biosensor and studied the performance of this sensor.The main work in this paper includes:(1) Vicinal diaminobenzoacridine (VDABA) was synthesized and characterized, The impact of reaction conditions (such as temperature, concentration, pH) on the NO detection of VDABA were studied, and it's standard curve when reacted with NO was detected. The feasibility and sensitivity of VDABA to use for biological detection was researched, including cytotoxicity testing and cell testing. The research indicated that it was suitable for the detection of NO in vivo.(2) A new NO sensitive material 9-(4-(1,2-diamine) benzene-N1-phenyl) acridine (DABPA) was prepared. The impact of reaction conditions (such as temperature, concentration, pH) on the NO detection of DABPA were studied, and its standard curve when reacted with NO were detected. The feasibility and sensitivity of DABPA to use for biological detection was researched, including cytotoxicity testing and cell testing. The research indicated that it was suitable for the detection of NO in vivo.(3) A new fluorescent NO fiber optic biosensor system was designed and constructed, VDABA was embedded in the NO-sensitive film and the conditions of this film was determined, while the performance of this sensor was studied, and the recovery test with serum was determined. Research showed that the detection range of this sensor for NO detection was 1.0×10-8～1.0×10-5 mol/L, the detection limit is estimated to be 1.0×10-9 mol/L, which has a very good linear relationship, and can be used for the accurate detection of animal serum.